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High-sensitivity human urine alpha2-macroglobulin detection kit

A detection kit and technology with high sensitivity, applied in the field of high-sensitivity human urine α2-macroglobulin detection kit, to achieve the effect of improving sensitivity and wide versatility

Inactive Publication Date: 2020-04-10
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, urine erythrocyte morphology examination and the average volume of erythrocytes in urine were commonly used to distinguish the two, but due to external factors such as indwelling specimens and the subjective consciousness of the tester, there are often limitations.

Method used

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  • High-sensitivity human urine alpha2-macroglobulin detection kit
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  • High-sensitivity human urine alpha2-macroglobulin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Preparation of α2-MG kit

[0046] Reagent R1 is a buffer solution containing PBS, pH=7.3±0.05 (25±0.5° C.).

[0047] Reagent R2 is a buffer solution containing BSA, pH=7.3±0.05 (25±0.5° C.).

[0048] The composition and pH of reagent R1 are shown in the table below.

[0049] PBS 150mM TRIS 20mM NaCl 1％ polyethylene glycol 10％ GENAPOL(R)X-080 0.05％ Proclin300 0.1％ pH 7.3±0.05(25±0.5℃)

[0050] The composition of reagent R2 is shown in the table below.

[0051] PBS 150mM antiserum 5％ BSA 0.1％ Proclin300 0.1％ pH 7.3±0.05(25±0.5℃)

[0052] Preparation of the calibrator: Add a certain amount of serum to it to make the concentration 100mg / L, and filter it with a 0.22um membrane to obtain the calibrator.

[0053] The composition and pH of the calibrator are shown in the table below.

[0054] PBS 150mM BSA 0.5％ Proclin300 0.1％ pH 7.3±0.05...

Embodiment 2

[0058] Embodiment 2: Preparation of α2-MG kit

[0059] Reagent R1 is a buffer solution containing TRIS-HCL, pH=7.5±0.05 (25±0.5° C.).

[0060] Reagent R2 is a buffer solution containing BSA, pH=7.5±0.05 (25±0.5° C.).

[0061] The composition and pH of reagent R1 are shown in the table below.

[0062] TRIS-HCL 300mM PBS 20mM NaCl 1.5％ polyethylene glycol 10％ GENAPOL(R)X-080 0.05％ Proclin300 0.1％ pH 7.5±0.05(25±0.5℃)

[0063] The composition and pH of reagent R2 are shown in the table below.

[0064]

[0065]

[0066] Preparation of the calibrator: Add a certain amount of serum to it to make the concentration 100mg / L, and filter it with a 0.22um membrane to obtain the calibrator.

[0067] The composition and pH of the calibrator are shown in the table below.

[0068] TRIS-HCL 300mM BSA 0.5％ Proclin300 0.1％ pH 7.5±0.05(25±0.5℃)

[0069] Preparation of quality control product: add a cer...

Embodiment 3

[0072] Embodiment 3: Preparation of α2-MG kit

[0073] Reagent R1 is a buffer solution containing HEPES, pH=7.3±0.05 (25±0.5° C.).

[0074] Reagent R2 is a buffer solution containing BSA, pH=7.3±0.05 (25±0.5° C.).

[0075] The composition and pH of reagent R1 are shown in the table below.

[0076] HEPES 200mM TRIS 10mM NaCl 1.2％ polyethylene glycol 8％ fatty acid polyoxyethylene ester 0.02％ Proclin300 0.1％ pH 7.3±0.05(25±0.5℃)

[0077] The composition and pH of reagent R2 are shown in the table below.

[0078] HEPES 200mM antiserum 5％ BSA 0.2％ Proclin300 0.1％ pH 7.3±0.05(25±0.5℃)

[0079] Preparation of the calibrator: Add a certain amount of serum to it to make the concentration 100mg / L, and filter it with a 0.22um membrane to obtain the calibrator.

[0080] The composition and pH of the calibrator are shown in the table below.

[0081] HEPES 200mM BSA 0.5％ ...

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Abstract

A high-sensitivity human urine alpha2-macroglobulin detection kit belongs to the field of detection kits and comprises a reagent R1, a reagent R2, a calibration product and a quality control product,wherein the reagent R1 contains a buffer solution, salt, an accelerant, an anti-interference agent and a preservative; the reagent R2 contains a buffer solution, antiserum, a stabilizer and a preservative; the calibration product contains a buffer solution, serum, a stabilizer and a preservative; the quality control product contains a buffer solution, serum, a stabilizer and a preservative; wherein the antiserum is anti-human alpha2-MG antiserum. The serum is human serum or animal serum. According to the invention, the sensitivity of the kit is improved by using a buffer solution compounding method; the content of alpha 2-macroglobulin in human urine is detected through immunoturbidimetry, a urine sample is directly tested on a machine without manual treatment, an anti-interference agent is added into the reagent, the sensitivity of the reagent is effectively improved, so that the test accuracy of the urine sample is improved, and the kit is suitable for various full-automatic biochemical analyzers and has wider universality.

Description

technical field [0001] The invention belongs to the technical field of detection kits, in particular to a high-sensitivity human urine α2-macroglobulin detection kit. Background technique [0002] α2-macroglobulin (α2-macroglobulin, α2-MG or AMG) is the protein with the largest molecular weight in plasma. The molecular weight is about 652,000-800,000, the sugar content is about 8%, and it is composed of 4 subunits. It is closely related to the development and function of lymphoreticular system cells. [0003] The most outstanding characteristic of α2-MG is that it can combine with various molecules and ions. In particular, it can combine with many proteolytic enzymes to affect the activity of these enzymes. Such as binding to many endopeptidases (including serine, sulfhydryl, carboxyl proteolytic enzymes and some metalloproteolytic enzymes). These proteolytic enzymes include plasmin, pepsin, chymotrypsin, trypsin and cathepsin D, etc. Studies have shown that the interac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/537
CPCG01N33/5375G01N33/6893G01N2800/347
Inventor 刘阳李慧婷潘晓恒张瑞张立媛夏冬梅孙成艳
Owner DIRUI MEDICAL TECH CO LTD
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