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A kind of method for rooting in a bottle of barley plum tissue culture seedling

A technology of tissue culture seedlings and wheat plums, applied in the field of plant tissue culture, can solve the problems of low survival rate and few high-quality commercial seedlings

Active Publication Date: 2022-04-01
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is commonly used for propagation by division or grafting. The rootstock is mountain peach, and cuttings are used for propagation. In summer, semi-mature branches are easy to survive, but the survival rate is not high, and there are few high-quality commercial seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0014] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0015] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.4mg / L, the seedlings grow root primordia in 7 days, the roots are 3~4cm long in 10 days, and the rooting rate is 95%. %.

[0016] The rooting medium also includes 26 g / L sucrose, 4 g / L agar powder, the pH of the medium is 5.8, the culture temperature is 22° C., the light time is 8 hours per day, and the light intensity is 1500 lx.

Embodiment 2

[0018] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0019] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0020] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.5mg / L, the seedlings grow root primordium in 8 days, the roots are 3~4cm long in 12 days, and the rooting rate is 97%. %.

[0021] The rooting medium also includes 27 g / L sucrose, 4.5 g / L agar powder, the pH of the medium is 5.9, the culture temperature is 23° C., the light time is 9 hours per day, and the light intensity is 2000 lx.

Embodiment 3

[0023] A method for rooting wheat plum tissue culture seedlings in a bottle, comprising the following steps:

[0024] (1) Selection of offspring seedlings: divide the offspring seedlings into individual plants, separate the height from the short ones, and select the offspring wheat and plum seedlings with a height of 3-4cm per plant, strong growth and stretched leaves;

[0025] (2) Rooting medium culture: transfer the selected secondary seedlings to the rooting medium 1 / 2WPM+IBA 0.6mg / L, the seedlings grow root primordia in 10 days, the roots are 3~4cm long in 12 days, and the rooting rate is 98%. %.

[0026] The rooting medium also includes 28 g / L sucrose, 5 g / L agar powder, the pH of the medium is 6.0, the culture temperature is 24° C., the light time is 10 hours per day, and the light intensity is 2500 lx.

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Abstract

The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rooting wheat plum tissue culture seedlings in a bottle. The present invention includes the selection of subcultured seedlings and the cultivation of rooting medium, wherein the selection criteria of the secondary seedlings are 3-4 cm in height per plant, strong growth, and stretched leaves, and the composition of the rooting medium includes 1 / 2WPM+IBA 0.4~0.8 mg / L, the present invention adopts a medium formula with a higher rooting rate, which effectively improves the rooting rate of rooting in the bottle of wheat plum tissue culture seedlings, and its rooting rate can reach 95%~100%, and the good product rate of wheat plum tissue culture seedlings High, the tissue culture method is easy to operate, can greatly reduce production costs, increase the propagation speed and uniformity of seedlings, and improve the stability of traits, accelerate the industrial production of wheat plums, and provide high-quality wheat plum seedlings for the market.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rooting wheat plum tissue culture seedlings in a bottle. [0002] technical background [0003] Mai Li, Rosaceae Prunus, deciduous shrub, up to 2 meters, leaves ovate-elliptic to elliptic-lanceolate, 5-8 cm long, apex acute and often rounded, base broadly cuneate, margins thin and blunt Teeth, glabrous on both sides or sparsely pubescent on the back of the middle rib; petiole 4-6mm long. The flowers are pink or nearly white, about 2 cm in diameter, and the pedicel is about 1 cm long. Fruit nearly spherical, 1-1.5 cm in diameter, red. Mai Li has a certain degree of cold resistance, strong adaptability, light-loving, relatively cold-resistant, strong adaptability, and is very beautiful. It is commonly cultivated and ornamental in gardens everywhere. It is suitable for lawn, roadside, rockery and forest edge cluster planting. Flower forcing an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周敬锋田星凯李涛
Owner WUXI MISHO ECOLOGY LANDSCAPE
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