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Vaccine strain screening method

A screening method and strain technology, applied in the field of screening, can solve the problems of limitation and lack of cross-protection effect, and achieve the effects of easy operation, shortened screening time, and short screening time.

Active Publication Date: 2020-05-26
WENS FOOD GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large number of RA serotypes (25 serotypes have been identified so far) and almost no cross-protection effect, only RA of the same serotype can effectively protect RA infection of the same serotype, and even RA of the same serotype is The protection provided by infection with the same serotype of RA is also different. How to screen the best strains from the same serotype of RA has many limitations in the commonly used methods of screening vaccine strains

Method used

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Examples

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Embodiment

[0040] In this embodiment, Riemerella anatipestifer (RA) is taken as an example to illustrate the screening method of the vaccine strain of the present invention. The screening process of the vaccine strain described in this embodiment is as follows: figure 1 shown. In this embodiment, 97 isolates of RA serotype I are used as materials to complete the screening of excellent vaccine strains. The specific screening method is as follows:

[0041] Step 1: Isolation, cultivation and identification of the strains to be tested

[0042] Take suspected diseased tissues such as liver and brain, dissect the diseased tissues in a sterile operating table, use an inoculation loop to stick the diseased materials containing bacteria, and draw a line on the surface of TSA solid medium containing 5% fetal bovine serum. The plates were placed in a constant temperature incubator at 37°C and 5% carbon dioxide for 24 hours.

[0043] Step 2: Serotyping

[0044] Pick a single colony from the plat...

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Abstract

The invention relates to a screening method, and concretely relates to a vaccine strain screening method. The vaccine strain screening method comprises the following steps: carrying out isolated culture on to-be-screened strains; identifying whether the to-be-screened strains are vaccine strains of required types or not, and if so, carrying out subsequent operation; identifying serotypes of the to-be-screened strains; obtaining the gene fingerprints of the to-be-screened strains; establishing a mixed strain infection model to obtain the optimal challenge dosage and the optimal time for separating the to-be-screened strains after challenge; screening winner strains and optimal winners; and preparing an inactivated vaccine from each strain in the optimal winners according to the same dosage,and immunizing to obtain the strain with the optimal protection effect, namely the required vaccine strain. The efficient vaccine strain screening method is short in screening time, easy to operate,convenient and rapid, greatly reduces the cost, and achieves the purpose of rapid screening.

Description

technical field [0001] The invention relates to a screening method, in particular to a screening method for vaccine strains. Background technique [0002] Vaccine strains are usually selected with high protection efficiency, small immunization dose, easy cultivation and low production cost. When the background knowledge of a certain bacterial research is vague and the virulence factors are not clear, the screening method of vaccine strains is usually to test the virulence of each isolate in turn, and different doses of bacteria are often used to infect susceptible animals. The lethal dose (MLD) and / or median lethal dose (LD50) can also be evaluated by minimal infectious dose (MID) and / or median infectious dose (ID50). These methods all require the determination of clinically isolated bacteria one by one, which requires a large workload, is time-consuming and laborious, and limits the number of strains that can participate in the screening. [0003] RA infection is a contag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689Y02A50/30
Inventor 刘军发邢娟娟周祺杨德鸿麦凯杰罗翠芬周庆丰刘士奇
Owner WENS FOOD GRP CO LTD
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