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Application of anti-ptn antibody in inhibiting leukemia stem cells and treating chronic myelogenous leukemia

An antibody and application technology, applied in the direction of antibody medical components, antibodies, anti-growth factor immunoglobulin, etc., can solve the problems of not being used to treat chronic myeloid leukemia, and the binding ability is not very ideal, and achieve the goal of inhibiting the growth of CML cells. Effect

Active Publication Date: 2021-01-01
碧燊控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, PTN antibodies have been mentioned in the US20040234519 patent, but on the one hand, their binding ability to PTN is not very satisfactory, on the other hand, it has not been used to treat chronic myeloid leukemia

Method used

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  • Application of anti-ptn antibody in inhibiting leukemia stem cells and treating chronic myelogenous leukemia
  • Application of anti-ptn antibody in inhibiting leukemia stem cells and treating chronic myelogenous leukemia
  • Application of anti-ptn antibody in inhibiting leukemia stem cells and treating chronic myelogenous leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Preparation of PTN antigen

[0028]The PTN cDNA was inserted into the pET-43.1b(+) vector to construct the PTN prokaryotic expression plasmid, which was designated as pET-43.1b(+)-PTN. Transform the above plasmids into Escherichia coli competent BL21 (DE3), then add the competent cells to 2ml LB culture containing ampicillin resistance for overnight culture, and inoculate 1% inoculum in 20 mL LB containing ampicillin resistance on the next day In LB medium, shake culture at 37 °C for 3 h, then add IPTG at a final concentration of 0.5 mmol / L and induce culture at 30 °C for 6 h. Collect the bacteria by centrifugation, add the lysate and perform ultrasonic crushing, collect the supernatant by centrifugation, and then further purify it through a nickel column to obtain the soluble recombinant antigen protein, digest it with enterokinase to remove the tagged protein carried on the plasmid, and finally obtain the PTN recombinant protein . Because the PTN protein ...

Embodiment 2

[0029] Example 2. Preparation of anti-PTN antibody

[0030] The PTN-KLH antigen prepared in Example 1 was injected intraperitoneally into female Balb / c mice together with Freund's complete adjuvant that caused an immune response, for initial immunization, and then injected into the above-mentioned females with the same amount of antigen every 2 weeks. Immunization was continued intraperitoneally in Balb / c mice. Finally, the last immunization was carried out 3 days before the fusion with myeloma cells. PTN-KLH antigen was mixed and emulsified with immune adjuvant, and injected intraperitoneally for the last immunization. The immunized Balb / c mouse splenocytes were taken and fused with the myeloma Sp2 / 0 cell line, and the fused cells were treated with Iscove medium (0.1 mM hypoxanthine, 0.4 μM aminopterin) containing 10% serum and 16 μM thymidine), diluted to an appropriate concentration, added to a 96-well plate for cultivation. After 10 days, the cell supernatant was taken, ...

Embodiment 3

[0035] Example 3. Determination of the binding ability of anti-PTN antibodies to PTN

[0036] The antibody plasmid obtained in Example 2 was transfected into CHO-K1 cells to screen for high-expression clones, and cultured in serum-free medium for expansion. Protein A affinity column was used to separate and purify the humanized antibody h5D7-2. The purified antibody was dialyzed with PBS to obtain the PTN humanized antibody h5D7-2 with high purity. The anti-PTN antibody 3B10 will be obtained through the US20040234519 patent, (for its light and heavy chain sequences, please refer to the appendix of the patent specification figure 1 B, the sequence of 1C) and the antibody 3B10 was obtained by the above method as a control antibody. The binding affinities of the humanized anti-PTN antibody h5D7-2 obtained above and the control antibody 3B10 to the PTN protein at 50% effective concentration (EC50) were evaluated by ELISA experiments. The recombinant human PTN protein was diluted...

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Abstract

The invention relates to an application of an anti-PTN antibody to inhibition of leukemia stem cells and treatment of chronic granulocytic leukemia, and the anti-PTN antibody can be combined with PAI-1 protein with high affinity, inhibit implantation of CML stem cells, and inhibit growth of CML stem cells. The antibody can be used for preventing or treating chronic granulocytic leukemia, and has awide application prospect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of anti-PTN antibody in inhibiting leukemia stem cells and treating chronic myelogenous leukemia. Background technique [0002] Chronic myelocytic leukemia (CML) is a malignant tumor formed by the abnormal proliferation of bone marrow hematopoietic stem cells. The onset is slow, and it is characterized by enlarged spleen and peripheral blood granulocytes. The translocation of chromosome 9 and chromosome 22 forms the Philadelphia chromosome (Ph), which then encodes the BCR-ABL1 fusion gene. Its specific biological markers eventually lead to the formation of leukemia stem cells (LSC) [1-3]. Because the BCR-ABL1 oncoprotein has continuously activated tyrosine kinase activity, it can induce the uncontrolled proliferation and apoptosis inhibition of leukemia cells, and cause the occurrence and development of chronic myeloid leukemia [4]. [0003] Currently, CML is mainly t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/22C12N15/85G01N33/68G01N33/574A61K39/395A61K31/506A61P35/02
CPCA61K31/506A61K39/39558A61P35/02C07K16/22C07K2317/52C07K2317/56C12N15/85G01N33/57426G01N33/68G01N2333/475A61K2300/00
Inventor 彭菲顾超
Owner 碧燊控股有限公司
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