A method for detecting short-chain fatty acids in serum and lymphoid tissue based on gc-ms
A technology for medium and short-chain fatty acids and lymphoid tissue, applied in the field of analytical chemistry, can solve the problems of complicated operation steps, low recovery rate results, high time cost, and achieve the effects of good repeatability and high recovery rate.
Active Publication Date: 2022-07-29
SHANDONG SHANWEI IMMUNOTECH CO LTD
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Problems solved by technology
[0004] The inventors found that there is currently no report on the quantitative detection method of short-chain fatty acids in lymphoid tissue. Although the quantitative method of short-chain fatty acids in serum has been reported, due to its cumbersome operation steps, high time cost, and low recovery results, etc. Features are not widely used
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Embodiment 1
[0040] (1) Instruments and reagents
[0041] Agilent 7890A gas chromatograph (Agilent Technology, USA); IKA VORTEX 3 vortex mixer (IKA, Germany); HH-S constant temperature water bath (Jintan Medical Instrument Factory, China); Refrigerated centrifuge (Thermo Fisher, USA) ); Milli-Q ultrapure water system (Millipore, USA).
[0042] Acetic acid, propionic acid, butyric acid, 2-ethylbutyric acid, pentafluorobromoben (Sigma-Aldrich, USA); Acetone, n-hexane (China Comeo Reagent Co., Ltd.); Phosphate buffer (China Dingguo Biological Reagent) company);
[0043] (2) Sample pretreatment
[0044] Serum: Leave the collected whole blood at room temperature for 30 minutes, centrifuge for 20 minutes at 4°C and 2000 rpm, and take the supernatant;
[0045] Lymphoid tissue: Accurately weigh 50mg of lymphoid tissue, add 1mL of pre-cooled ultrapure water, use a tissue grinder to grind evenly at 4°C, vortex vigorously for 1min, extract by ultrasonic for 15min, centrifuge at 4°C 10000rpm for 10...
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Abstract
The invention discloses a method for detecting short-chain fatty acids in serum and lymphoid tissue based on GC-MS, comprising the following steps: preprocessing lymphoid tissue to obtain a lymphatic extract; adding an internal standard substance to the serum or lymphatic extract, And adding pentafluorobromobenzine to carry out derivatization reaction; after the derivatized solution is extracted, acetic acid, propionic acid and butyric acid in the extract are detected by GC-MS. The invention establishes a gas chromatography-mass spectrometry rapid determination method for detecting three kinds of short-chain fatty acids in serum and lymphoid tissue through a derivatization method. Pre-process and derivatize different biological samples, and add internal standard for correction to achieve accurate quantification.
Description
technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for detecting short-chain fatty acids in serum and lymphoid tissue based on GC-MS. Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Short chain fatty acids (Short chain fatty acids), also known as volatile fatty acids, according to the number of carbon atoms, organic fatty acids with a carbon number of 1-6 are called short chain fatty acids, among which acetic acid, propionic acid and butyric acid are due to Its potential biological effects have attracted much attention. High concentrations of short-chain fatty acids in the intestinal tract can regul...
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045G01N2030/067
Inventor 宋月曲新艳王泉博
Owner SHANDONG SHANWEI IMMUNOTECH CO LTD