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Method for determination of bentazone residues in plant-derived foods by liquid chromatography-mass spectrometry/mass spectrometry

A liquid chromatography and mass spectrometry technology, applied in the field of detection of pesticide residues in food, can solve the problem of high detection limit, and achieve the effect of reducing the detection limit, high recovery rate and optimizing the pretreatment method.

Active Publication Date: 2022-07-01
哈尔滨海关技术中心
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Problems solved by technology

[0003] The purpose of the present invention is to solve the technical problem that the detection limit of existing methods for detecting bentazone residues is relatively high, and to provide a method for measuring bentazone residues in plant-derived foods by liquid chromatography-mass spectrometry / mass spectrometry

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  • Method for determination of bentazone residues in plant-derived foods by liquid chromatography-mass spectrometry/mass spectrometry
  • Method for determination of bentazone residues in plant-derived foods by liquid chromatography-mass spectrometry/mass spectrometry
  • Method for determination of bentazone residues in plant-derived foods by liquid chromatography-mass spectrometry/mass spectrometry

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specific Embodiment approach 1

[0089] Embodiment 1: The method for determining the residual amount of bentazone in plant-derived food by liquid chromatography-mass spectrometry / mass spectrometry in this embodiment is carried out according to the following steps:

[0090] 1. Take 500g samples of apple, tomato, kidney bean, radish, cabbage, shiitake mushroom or onion, chop the edible part of the sample, process the sample into a slurry with a masher, mix well, and store it in a freezer below -18°C. to obtain a uniform sample;

[0091] 2. Extraction:

[0092] Weigh 5g of uniform sample (accurate to 0.01g), put it in a 50mL centrifuge tube, add 0.3mL of hydrochloric acid solution with a concentration of 1mol / L, 20mL of acetonitrile, and homogenize it with a homogenizer at 15 000r / min for 2min, at 5 After centrifugation at 000 r / min for 5 min, the supernatant was transferred to a 50 mL volumetric flask, and the residue was repeatedly extracted with 20 mL of acetonitrile. Combine the extracts in the same volumet...

specific Embodiment approach 2

[0126] Specific embodiment 2: The method for determining the residual amount of bentazone in plant-derived food by liquid chromatography-mass spectrometry / mass spectrometry in this embodiment is carried out according to the following steps:

[0127] 1. Take 500g of rice, corn, soybean or tea samples, pulverize with a pulverizer, mix well, and store at room temperature to obtain a uniform sample;

[0128] 2. Extraction:

[0129] Weigh 5g (accurate to 0.01g) of a uniform sample (weigh 1g for a tea sample), place it in a 50mL centrifuge tube, add 0.3mL hydrochloric acid solution with a concentration of 1mol / L, 10mL water, soak for 30min, add 20mL acetonitrile, use a homogeneous After homogenizing at 15 000 r / min for 2 min, centrifuge at 5 000 r / min for 5 min, transfer the supernatant to a 50 mL volumetric flask, repeat the extraction with 20 mL of acetonitrile, and combine the extracts in the same volumetric flask, And dilute to 50mL with water, pipette 10mL of the extract, adju...

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Abstract

A liquid chromatography-mass spectrometry / mass spectrometry method for determining the residues of bentazone in plant-derived foods relates to a detection method for pesticide residues in food, and belongs to the field of pesticide residue detection. The purpose of the present invention is to solve the technical problem of the high detection limit of the existing methods for detecting bentazone residues. The method is as follows: 1. Prepare a uniform sample; 2. Extraction; 3. Purify; 5. Quantitative determination; 6. Blank test; 7. Calculation and presentation of results, that is, the residues of bentazone in food. The method of the invention changes the gas chromatography of the existing method into liquid chromatography-mass spectrometry / mass spectrometry, optimizes the pretreatment method, and reduces the detection limit of the method. The detection limit of the method is 10 μg / kg, and the The recovery rate of pine is high.

Description

technical field [0001] The invention relates to a detection method for pesticide residues in food, and belongs to the field of pesticide residue detection. Background technique [0002] After my country's entry into the WTO, the technical barriers to import and export of WTO member countries will become more and more strict. Therefore, it is necessary to strengthen the study of WTO rules and know ourselves and others. The inspection and quarantine system and relevant departments should strengthen information communication and guidance on testing techniques. Start from the source, seriously implement the pesticide residue monitoring plan, and prohibit the use of high-toxicity and high-residue pesticides. In the existing method, SN / T 0292-2010 "Gas Chromatography Method for Detection of Residues of bentazone in cereals for export" is used to detect the residues of bentazone in cereals, but the detection limit of this detection method is relatively high. SUMMARY OF THE INVEN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/14G01N30/06G01N30/8634G01N2030/146
Inventor 魏冬旭姜冰吴岩韩广源勾越刘鹏
Owner 哈尔滨海关技术中心
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