A method for detecting black carbon in animal biological samples
A detection method and biological technology, which is applied in the field of atmospheric particle poison research, can solve the problems of high instrument cost, lack of material structure information, poor promotion of detection technology, etc., and achieve the effect of improving the accuracy of data and strong promotion
Active Publication Date: 2021-11-16
XIAMEN UNIV
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In the past two years, although foreign studies have studied black carbon exposure from the level of biological monitoring, the detection technology is limited to characterizing the light absorption characteristics and morphology of black carbon, lacking material structure information at the molecular level ([9] Saenen, N.D. et al .Children's urinary environmental carbon load: A novel marker reflecting residential ambient air pollution exposure.Am.J.Respir.Crit.Care.Med.2017,196,873–881); and the instrument cost of this detection method is high, resulting in poor promotion of detection technology, It is urgent to develop a black carbon detection method with low cost, wide applicability, simple operation and easy promotion
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0035] This example design is based on the detection of black carbon concentration in the blood of mice exposed to PM 2.5 in the real environment. The specific steps are as follows:
[0036] 1. Exposure experiment of mice in actual environment PM2.5
[0037] Atmospheric fine particle sampler is used to trap PM2.5 particles on the filter membrane, and the obtained particle samples and buffer solution (phosphate buffer or saline) are ultrasonically prepared into a suspension with a certain concentration for tracheal instillation, PM2 .5 The concentration of the suspension is converted according to the Chinese air quality standard and the average body weight of the mice. Mice were exposed to atmospheric particulate matter by tracheal instillation after a period of adaptive feeding.
[0038] 2. Nitrolysis of mouse blood samples
[0039] After one experimental period of acute exposure, blood from mice was added to concentrated nitric acid at a ratio of 1:4, then put into a Teflon...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for detecting black carbon in animal biological samples, which relates to the research field of atmospheric particulate poisons. Collect animal biological samples, decompose the black carbon particles in the samples into small molecular benzene polycarboxylates, use gas chromatography-mass spectrometry platform to detect the concentration of benzene polycarboxylates, and finally convert the concentration of benzene polycarboxylates into black carbon concentration. Using strong acidic conditions to maintain the molecular state of benzene polycarboxylic acid in aqueous solution, the adsorption of target molecules is achieved based on the mechanism of π-π adsorption between molecular state benzene polycarboxylic acid and the stationary phase, and the operation of strong acidity Conditions favor dechelation and elution of interferents such as metal ions. Using this solid-phase extraction technology greatly simplifies the process of extracting benzene polycarboxylic acid in black carbon nitrification solution, and finally detects the concentration of derivatized benzene polycarboxylic acid through gas chromatography-mass spectrometry platform and then converts it into the concentration of black carbon. Make up for the shortcomings of the previous benzene polycarboxylic acid method that can only analyze black carbon in environmental samples.
Description
technical field [0001] The invention relates to the field of atmospheric particle poison research, in particular to a method for detecting black carbon in animal biological samples. Background technique [0002] With the rapid development of my country's economy, my country's industrialization and urbanization are accelerating, and the total amount of pollutant discharge is high all the year round. Air pollution has become an inevitable and serious problem that affects the health of residents. The main particulate air pollutants that affect the health of residents are PM 2.5 (particles with an aerodynamic diameter less than 2.5 microns) that can enter the lungs, which are mainly produced through fuel combustion, transportation, industrial production, construction and road dust, etc., while black Carbon is again the key component of PM 2.5. [0003] Relevant epidemiological studies have shown that black carbon exposure is associated with low birth weight in early life ([1] P...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 申河清潘虹张洁
Owner XIAMEN UNIV