Method for detecting black carbon in animal biological sample
A detection method and biological technology, applied in the field of atmospheric particle poison research, can solve the problems of lack of material structure information, high cost of instruments, poor popularization of detection technology, etc., and achieve the effect of strong popularization.
Active Publication Date: 2020-06-19
XIAMEN UNIV
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Problems solved by technology
In the past two years, although foreign studies have studied black carbon exposure from the level of biological monitoring, the detection technology is limited to characterizing the light absorption characteristics and morphology of black carbon, lacking material structure information at the molecular level ([9] Saenen, N.D. et al .Children's urinary environmental carbon load: A novel marker reflecting residential ambient air pollution exposure.Am.J.Respir.Crit.Care.Med.2017,196,873–881); and the instrument cost of this detection method is high, resulting in poor promotion of detection technology, It is urgent to develop a black carbon detection method with low cost, wide applicability, simple operation and easy promotion
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Embodiment 1
[0035] This example is designed to detect the concentration of black carbon in the blood of mice exposed to PM 2.5 in the real environment. The specific steps are as follows:
[0036] 1. PM2.5 exposure experiment in the actual environment of mice
[0037] An atmospheric fine particle sampler is used to trap PM2.5 particles on the filter membrane, and the obtained particle samples are ultrasonically prepared with a buffer solution (phosphate buffer or normal saline) to prepare a suspension of a certain concentration for tracheal instillation. PM2 .5 The concentration of the suspension is converted according to Chinese air quality standards and the average body weight of mice. Mice were exposed to atmospheric particulate matter by tracheal instillation after a period of adaptive feeding.
[0038] 2. Nitrolysis of mouse blood samples
[0039] After an experimental period of acute exposure, the blood of mice was added with concentrated nitric acid at a ratio of 1:4 and then plac...
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The invention discloses a method for detecting black carbon in an animal biological sample, and relates to the field of atmospheric particle poison research. The method comprises the following steps:collecting an animal biological sample, carrying out nitrolysis on black carbon particles in the sample to obtain small-molecular-state benzene polycarboxylic acid, detecting the concentration of thebenzene polycarboxylic acid by using a gas chromatography-mass spectrometry platform, and finally converting the concentration of the benzene polycarboxylic acid into the concentration of black carbon. Molecular state of benzene polycarboxylic acid in an aqueous solution is maintained by utilizing a strong acidic condition, adsorption of target molecules is realized based on an action mechanism taking pi-pi adsorption as a main component between the molecular state benzene polycarboxylic acid and a stationary phase, Meanwhile, the strong acidic operation condition is beneficial to dechelationand elution of interferents such as metal ions and the like. By utilizing the solid-phase extraction technology, the process of extracting the benzene polycarboxylic acid in the black carbon nitrolysis solution is greatly simplified, and finally, the concentration of the derivatized benzene polycarboxylic acid is detected through a gas chromatography-mass spectrometry platform and is converted into the concentration of the black carbon. And the defect that the conventional benzene polycarboxylic acid method can only analyze black carbon in an environmental sample is overcome.
Description
technical field [0001] The invention relates to the field of atmospheric particle poison research, in particular to a method for detecting black carbon in animal biological samples. Background technique [0002] With the rapid development of my country's economy, my country's industrialization and urbanization are accelerating, and the total amount of pollutant discharge is high all the year round. Air pollution has become an inevitable and serious problem that affects the health of residents. The main particulate air pollutants that affect the health of residents are PM 2.5 (particles with an aerodynamic diameter less than 2.5 microns) that can enter the lungs, which are mainly produced through fuel combustion, transportation, industrial production, construction and road dust, etc., while black Carbon is again the key component of PM 2.5. [0003] Relevant epidemiological studies have shown that black carbon exposure is associated with low birth weight in early life ([1] P...
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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 申河清潘虹张洁
Owner XIAMEN UNIV