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A Breeding Method for Quickly Establishing Early Flowering Resources of Brassica napus

A cabbage type and spring rapeseed technology is applied in the field of rapidly shortening the growth period of cabbage type rapeseed and creating excellent early-flowering resources, which can solve the problems of large workload and low efficiency of breeding methods, avoid phenotypic selection, improve selection efficiency, The effect of shortening the breeding cycle

Active Publication Date: 2022-05-10
QINGHAI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: how to provide a breeding method for quickly creating early flowering resources of Brassica oleracea, and solve the problems of heavy workload and low efficiency of existing breeding methods

Method used

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  • A Breeding Method for Quickly Establishing Early Flowering Resources of Brassica napus
  • A Breeding Method for Quickly Establishing Early Flowering Resources of Brassica napus
  • A Breeding Method for Quickly Establishing Early Flowering Resources of Brassica napus

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Embodiment 1

[0070] A method for rapidly creating spring Brassica napus resources, comprising the following steps:

[0071] (1) Using the developed molecular markers closely linked to the early flowering QTL: G1803 and IA7-4 closely linked to the main early flowering QTL cqDTFA7a, S035 and SNP11 closely linked to the main early flowering QTL cqDTFC8 to identify 93 cabbage Type spring rape flowering genotype, construct early flowering genotype library;

[0072] The specific method is as follows: the method for screening a single plant containing the cqDTFA7a site is as follows: using Brassica napus DNA as a template, respectively using the primer pair shown in SEQ ID No.1 and SEQ ID No.2, and the primer pair shown in SEQ ID No.3 Perform PCR amplification with the primer pair shown in SEQ ID No.4, if the amplified products of 330bp and 120bp can be obtained respectively, then the Brassica napus is a single plant containing the cqDTFA7a site;

[0073] The method for screening a single plant ...

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Abstract

The invention discloses a breeding method for quickly creating early flowering resources of Brassica oleracea, (1) using four molecular markers to screen out Brassica napus strains containing cqDTFA7a and cqDTFC8 sites respectively, (2) A single plant containing the cqDTFA7a locus and a single plant containing the cqDTFC8 locus were used for hybridization. (3) The F1 generation of the cross was cultured for microspores. The DH strains that doubled successfully were genotyped using 4 molecular marker primer pairs, and screened out For DH single plants containing both cqDTFA7a and cqDTFC8 loci, (4) identify the yield and flowering period of the screened DH single plants, and screen out single plants with excellent yield and flowering period 3 days earlier than the parent. Through molecular marker-assisted selection of target trait genes, the loss of target trait genes in the process of cross breeding can be avoided, the selection efficiency can be improved, and the breeding cycle can be shortened and the workload can be reduced.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and relates to a method for rapidly shortening the growth period of Brassica napus and creating excellent early flowering resources. Background technique [0002] Rapeseed (Brassica campestris L.) is a plant of the genus Brassica in the family Brassicaceae. It is one of the main oil crops in the world, and it is also one of the main oil crops in China. Its planting area accounts for 54% of the total planting area of ​​oil crops in the country. , while China's rapeseed area accounts for 20% of the world's rapeseed area. Brassica napus L. (AACC, n=19) originated in Europe and is one of the three major types of rape. A composite species that evolved from double diploidization after hybridization. Brassica napus has a short growth period and short individuals, and is mainly distributed in high-altitude areas with harsh climate conditions and short frost-free periods; Brassica napus has hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04A01H6/20
CPCC12Q1/6895A01H1/02A01H1/04A01H6/20C12Q2600/13C12Q2600/156
Inventor 柳海东杜德志
Owner QINGHAI UNIVERSITY
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