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A method for developing siRNA for covid-19 virus drug treatment

A technology for COVID-19 and viruses, which is applied in the field of siRNA research and development for COVID-19 virus drug treatment, and can solve problems such as the inability to quantitatively evaluate the efficiency of candidate siRNA sequences

Active Publication Date: 2020-12-22
JILIN UNIV
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Problems solved by technology

[0005] The main purpose of the present invention is to solve the problem that the rule-based design method cannot quantitatively evaluate the efficiency of candidate siRNA sequences;

Method used

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  • A method for developing siRNA for covid-19 virus drug treatment
  • A method for developing siRNA for covid-19 virus drug treatment

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Embodiment Construction

[0095] see Figure 1 to Figure 2 Shown:

[0096] The siRNA research and development method for COVID-19 virus drug treatment provided by the present invention, its method is as follows:

[0097] The siRNA research and development method includes two parts: preliminary screening and machine learning model prediction and selection. The specific steps are as follows:

[0098] The first part: the preliminary screening of potential high-efficiency siRNA based on multiple indicators, the specific steps are as follows:

[0099] Step 1. Select the S gene sequence in the COVID-19 virus genome as the target sequence, and perform multiple sequence comparison analysis on the genomes of all other mutant strains of the COVID-19 virus to determine the conserved region of the target target sequence;

[0100] Step 2, search the gene coding sequence of the conserved region of the target target sequence for a 19nt long nucleotide subsequence, and obtain the corresponding siRNA duplex according...

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Abstract

The invention discloses a research and development method of siRNA for COVID-19 virus drug therapy, which comprises: a first part of preliminarily screening potential high-efficiency siRNA based on multiple indexes, which specifically comprises the following steps: step 1, selecting an S gene sequence as a target sequence; step 2, obtaining a corresponding siRNA double strand; step 3, screening asiRNA sequence with a concentration of 36 to 53%; step 4, screening out siRNA with free energy; step 5, defining and calculating an index I; step 6, screening out siRNA of the first 50%; step 7, defining and calculating an index II; step 8, screening out siRNA with the index II being equal to 5; step 9, defining and calculating an index III; step 10, ranking the first 50% of siRNA; step 11, directly taking all the candidate siRNAs selected in the step 10; step 12, specifically targeting a target sequence; step 13, taking the remaining siRNA as the siRNA subjected to preliminary screening; anda second part of performing interference efficiency prediction and optimization by using a machine learning model. The method has the beneficial effect of realizing safe, reliable and high-interference-efficiency siRNA design.

Description

technical field [0001] The present invention relates to an siRNA research and development method for drug treatment, in particular to a siRNA research and development method for COVID-19 virus drug treatment. Background technique [0002] At present, RNA interference technology is an effective gene research tool developed in recent years. Its wide application has accelerated the pace of functional genomics research and also promoted research in related fields such as gene therapy. A key factor affecting the efficiency of RNA interference is the design of the siRNA sequence. RNA interference technology requires that the siRNA sequence and the mRNA sequence at the target site must be strictly matched, and a single base mismatch may cause RNA interference to fail. Therefore, designing an effective siRNA sequences can increase the efficiency of gene silencing. A large number of experiments have shown that the effect of siRNA designed for the same target mRNA varies greatly, bec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B40/00G16B30/00G16B15/30
CPCG16B15/30G16B30/00G16B40/00
Inventor 朱晓冬陈梦欣刘元宁张浩
Owner JILIN UNIV
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