Method of combined detection for diagnosis of breast cancer by adopting ddPCR technology
A technology for joint detection and breast cancer, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as difficulty in detection, inability to meet tumor molecular typing, and inability to detect early in situ breast cancer, etc. To achieve the effect of accurate data, reliable results, and auxiliary judgment
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[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work, all belong to the protection scope of the present invention.
[0037] see Figure 1-2 , the embodiment of the present invention provides a kind of technical scheme: a kind of method that utilizes ddPCR technology to jointly detect and diagnose breast cancer, specifically comprises the following steps:
[0038] S1. Extract genomic DNA and miRNA in the sample: use a commercial genome extraction kit to extract the total DNA in the sample, then use a commercial miRNA extraction kit to extract the total mi...
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