Method of combined detection for diagnosis of breast cancer by adopting ddPCR technology

The invention discloses a method of combined detection for diagnosis of a breast cancer by adopting a ddPCR technology. The method specifically comprises the following steps of S1, extracting genomicDNA and miRNA from a sample; S2, carrying out modification and purification quantification through DNA sulfite and reversely transcribing the miRNA into cDNA; S3, separately carrying out ddPCR by employing purified DNA genome as a template and a specific primer as a primer for detecting a methylation state of MASPIN; and S4, calculating methylation degree W of an upstream regulatory region CpG island of a maspin coding sequence of a to-be-detected sample and combining the methylation degree W with the content of miR-142 in the sample for analysis. The invention relates to the technical field of biology and disease diagnosis. According to the method of combined detection for diagnosis of the breast cancer by adopting the ddPCR technology, combined detection of two indicators, namely the methylation state of the maspin gene and the content of the miR-142 can be used for evaluating whether a patient suffers from the breast cancer or not, the data are more accurate, the result is more reliable, and the method can be widely applied to early screening for the breast cancer and effectively assists judgement the malignant degree and metastasis of the breast cancer.