A rapid and effective non-invasive assay for assessing the outcome of in vitro fertilization

Abstract

The invention provides a non-invasive detection method for quickly and effectively evaluating the outcome of in vitro fertilization, which comprises the following steps: blending the obtained sperm into the culture fluid; dripping the prepared pre-fertilization culture fluid into the culture dish, Prepare a plurality of pre-fertilization culture drops in a petri dish; inject the single ovum taken out into each pre-fertilization culture drop respectively, and cultivate for 4-5 hours; absorb part of the fertilization drop in the fertilization drop, centrifuge, and take The supernatant was diluted; the diluted fertilized drops were detected with an automatic biochemical analyzer for calcium ion concentration; the difference in calcium ion concentration in the fertilized drops was calculated to predict the difference in OPN outcome and / or 2PN outcome. In this study, the fertilization drops (Day 0) that were exchanged for the first 4-5 hours were collected for early detection. The results of the study can directly predict the fertilization outcome of the 2PN stage (Day 1), which will help improve the early clinical judgment of the fertilization outcome and provide a basis for further diagnosis and treatment. Provide guidance.

Description

technical field [0001] The invention relates to clinical assisted reproductive technology, in particular to a non-invasive detection method for quickly and effectively evaluating the outcome of in vitro fertilization. Background technique [0002] In vitro fertilization and embryo transfer (IVF-ET) is commonly known as "test-tube baby". It is a special clinical assisted reproductive technology, which needs to complete the fertilization process of sperm and eggs in an in vitro environment, and then transfer the fertilized early embryos into the female uterus, and develop into a fetus in the female uterus, which is commonly known as test tube baby. In this process, whether to fertilize is the first and most critical step. If the fertilization outcome can be quickly and accurately assessed, remedial measures can be given to the patient at the best time, thereby reducing the mental and economic pressure of the patient. At present, the main method of clinical evaluation of fert...

AI Technical Summary

Problems solved by technology

Embryo experimenters mainly conduct morphological evaluation by microscopic observation, which is greatly affected by the subjectivity of the observer, and cannot detect some hidden abnormalities that cannot be seen with the naked eye. Embryos with high morphological scores cannot represent the best developmental potential. Therefore, the prediction of IVF outcome is limited

Images