Application of zona block-based ovastacin protein in rapid non-invasive detection of in vitro fertilization

Abstract

The present invention provides an application of zona block-based ovastacin protein in rapid non-invasive detection of in vitro fertilization, by detecting the ovastacin protein released from oocyte zona block in fertilized droplets, early and accurately judging whether the fertilization is successful or not. The present invention is based on the detection of the specificity of the ovastacin protein released by the zona block mechanism of the oocyte during the fertilization process. The protein is released from the oocyte only after the oocyte is successfully fertilized to prevent polyspermy. By detecting ovastacin protein to determine whether the oocyte was fertilized successfully. Compared with the traditional method of egg removal by scientific researchers, this method is more objective and the detection time is relatively early. At this time, the oocytes have not yet aged, and then quickly determine whether ICSI is needed for remediation. The detection method is non-invasive and suitable for large-scale Promote apps.

Description

technical field [0001] The invention belongs to the technical field of reproduction, and in particular relates to the application of zona block-based ovastacin protein in rapid non-invasive detection of in vitro fertilization. Background technique [0002] In vitro fertilization-embryo transfer technology uses drugs to make a batch of women's eggs grow and mature synchronously. After being taken out by puncture, they are placed in a petri dish with her husband's optimized sperm for co-incubation. After a period of in vitro fertilization, they develop into fertilized eggs. , and then gradually develop into embryos by cleavage; then select high-quality embryos and place them in the woman's uterus, and finally develop into fetuses. [0003] There are currently two main methods of in vitro fertilization in assisted reproduction, namely long-term fertilization and short-term fertilization. Long-term fertilization is to judge whether the fertilization is successful by observing t...

AI Technical Summary

Problems solved by technology

In prolonged fertilization methods, failure to fertilize will not be remedied as eggs are already aged after 17-19 hours ICSI remediation is poor

There are many shortcomings in the observation of the second polar body in the short-term fertilization method, which may easily lead to misjudgment

For example: after the polar body is discharged, it is easy to break and cannot be observed; theoretically, the first polar body may be split into two polar bodies, or broken into two parts, which will be misjudged as a first polar body and a second polar body Diodes, which are misjudged as successful fertilization or egg removal, or other fragments similar to polar bodies may be produced in the zona pellucida during the culture process, which will be misjudged as polar bodies, etc.