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Artificial reproduction-hybridization joint breeding method for meat goat

A technology of artificial breeding and goats, applied in biochemical equipment and methods, germ cells, microorganisms, etc., can solve the problems of restricting the selection of new breeds/lines of meat goats, immature technology, etc., and achieve a good survival rate of hybrid blastocysts , high fertilization potential, and the effect of improving the survival rate

Inactive Publication Date: 2020-07-14
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology is currently less used in meat goat breeding, and the technology is still immature, which limits the selection of new breeds / lines of meat goats

Method used

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  • Artificial reproduction-hybridization joint breeding method for meat goat
  • Artificial reproduction-hybridization joint breeding method for meat goat
  • Artificial reproduction-hybridization joint breeding method for meat goat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of artificial propagation-hybrid combined breeding method of meat goats, comprising the following steps:

[0027] S1, Lvliang black goat with good meat performance was selected as the female parent, and Jintang black goat with a large body was selected as the male parent;

[0028] S2, (1) Treat the female parent with ovulation induction in a conventional way (for ovulation induction methods, refer to Zhao Yongju, Sun Xinming, He Peng, et al. Comparison of the effects of superovulation treatment on ewes of different parity[J]. Animal Science, 2005(s1).), the isolated maternal oocytes were cultured in vitro to mature oocytes containing the first polar body, and a total of 13 mature oocytes were collected for future use.

[0029] The method of collecting mature oocytes is as follows: wash the isolated ovary with sterilized physiological saline containing penicillin-streptomycin mixture for 3 times, put it in 37°C physiological saline to keep warm, and use a 10mL syr...

Embodiment 2

[0042] A kind of artificial propagation-hybrid combined breeding method of meat goat, the operation is basically the same as that of embodiment 1, the difference is:

[0043] The prescription of described sperm motility enhancing liquid is as follows: Embryo culture water 97ml, MgSO 4 ·7H 2 O 0.02g, KCl 0.05g, streptomycin 0.005g, penicillin 0.006g, NaHCO 3 0.04g, sodium pyruvate 0.004g, CaCl 2 2H 2 O 0.02g, FBS 3ml, Hepes 0.5g.

[0044] The formula of the sperm supernatant is as follows: 100ml of embryo culture water, MgSO 4 ·7H 2 O 0.03g, KCl 0.06g, NaHCO 3 0.05g, sodium pyruvate 0.005g, CaCl 2 2H 2 O 0.02g.

[0045] The freezing-thawing treatment step is carried out before the fertilized eggs are cultured for hybrid blastocysts, and the specific conditions are: the fertilized eggs are cooled from room temperature 20°C at 0.5°C / min to -15°C, and then heated at 0.5°C / min to room temperature 20°C, Then carry out hybrid blastocyst culture, this step can eliminate th...

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Abstract

The invention belongs to the field of animal breeding and particularly relates to an artificial reproduction-hybridization joint breeding method for a meat goat. The method comprises the steps of selecting one goat variety with good meat performance as a female parent, selecting another goat variety with a large body size as a male parent; culturing oocytes of the female parent in vitro into mature oocytes containing a first polar body, and soaking semen of the male parent with a semen motility enhancement liquid and then preparing a semen suspension; carrying out in vitro fertilization on themature oocytes and the semen suspension, culturing zygotes into a hybrid blastocyst form, transplanting hybrid blastocysts into a body of the female parent for developing to natural delivery in the body of the female parent and obtaining hybrid F1; and backcrossing the hybrid F1 and the female parent, backcrossing backcross descendants and the female parent for multiple times until a breeding objective is met, and carrying out crossbred fixing to obtain a new variety / line with good characters. According to the method, an artificial assistant reproduction technology is combined with a traditional hybridization technology, so that the number of animal embryos can be increased, the survival rate of the animal embryos can be improved and the method has a good application prospect in the fieldof animal breeding.

Description

technical field [0001] The invention belongs to the field of animal breeding, and in particular relates to an artificial propagation-hybrid combined breeding method of meat goats. Background technique [0002] Good breeds / lines of livestock are the basis for efficient animal husbandry. For mutton sheep, their fecundity, meat production and meat quality directly determine the level of economic benefits. Therefore, it is particularly important to cultivate new breeds / lines of mutton sheep that meet current social needs. Crossbreeding is a commonly used method for breeding new varieties / lines. It obtains stable offspring with comprehensive traits by crossing the male and female parents with excellent traits. However, due to the limitation of the number of embryos in the animal breeding process, each crossing produces The number of progeny is very limited, resulting in a long breeding time, unable to meet the breeding production requirements in time. [0003] Artificial assis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02C12N5/075C12N5/076
CPCA01K67/02C12N5/0609C12N5/061C12N2500/16C12N2500/12C12N2500/14C12N2500/30
Inventor 刘建华乔利英刘文忠程俐芬高晋生安丽霞李宝钧刘巧霞
Owner SHANXI AGRI UNIV
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