Culture medium and method for rapidly inducing seeds of brachypodium distachyon to generate calluses

A technology of Brachypodium erinaceus and callus, which is applied in the field of plant tissue culture and can solve the problems of insufficient time for plant regeneration and the like

Inactive Publication Date: 2020-07-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, this technology has been widely used in plant regeneration, but the research on how to shorten the time of plant regeneration is still not in-depth, especially how to quickly induce good callus formation

Method used

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  • Culture medium and method for rapidly inducing seeds of brachypodium distachyon to generate calluses
  • Culture medium and method for rapidly inducing seeds of brachypodium distachyon to generate calluses

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Embodiment 1

[0038] A medium for rapidly inducing callus from Brachypodium distachyon seeds, including MS basic medium+100uMFPX+0.6mg / L CuSO 4 . Fipexide (FPX) is a novel chemical inducer.

[0039] The compound method of described 100uM FPX is: 388.84mg is dissolved in 5mL DMSO solution, after stirring and dissolving, make up to 10mL with distilled water, obtain 100mM FPX mother liquor, get 100mM FPX mother liquor of 1ml and add in 1L culture medium to obtain 45-100uM FPX .

[0040] The 0.6mg / mL CuSO 4 The preparation method is: 50mg CuSO 4 Dissolve 5H2O in 50mL distilled water to obtain 1mg / mL CuSO4, store in the dark at 4°C, take 600ul 1mg / mL CuSO 4 into 1L medium to prepare CuSO with a final concentration of 0.6mg / L 4 .

[0041] 6. The preparation method of 25% sodium hypochlorite solution: absorb 12.5mL sodium hypochlorite and dissolve in 180mL distilled water, and make up to 200ml with distilled water.

[0042] The preparation method of 1M KOH: 5.6g KOH solid was dissolved in 1...

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Abstract

The invention discloses a culture medium and a method for rapidly inducing seeds of brachypodium distachyon to generate calluses. Filexide (FPX) and CuSO4 are added to an MS basic culture medium. Theseeds of brachypodium distachyon are cut by a surgical knife to break dormancy of the seeds, the rapid calluses of the seeds of brachypodium distachyon are obtained under the synergistic action of FPX, and the induction rate is as high as 98.3%. The culture medium and method can be directly used for induction of the calluses of the seeds of brachypodium distachyon, shorten time of plant tissue culture, and lay a foundation for accelerating plant transgenic breeding.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a medium and a method for rapidly inducing callus from Brachypodium distachyon seeds. Background technique [0002] An effective way to study plant gene function through plant tissue culture technology. At present, this technology has been widely used in plant regeneration, but the research on how to shorten the time of plant regeneration is still not in-depth, especially how to quickly induce good callus formation. [0003] Brachypodium distachyon, as the most widely distributed C3 plant in the world, has been defined as a new model plant because of its small genome, short growth cycle, small size, and suitability for genetic transformation, laboratory and field operations. At the same time, Brachypodium distachyon and wheat belong to the subfamily Poaaceae, and the seeds of this subfamily have obvious dormancy phenomenon, and the seeds need to go through a certain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/00
Inventor 王凯余光润王建勇苗丽
Owner FUJIAN AGRI & FORESTRY UNIV
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