SSR primer group of taro and application thereof

A technology of primer set and primer pair, which is applied in the direction of recombinant DNA technology, measurement/testing of microorganisms, biochemical equipment and methods, etc. It can solve the problems of insufficient number of SSR primers, low and insufficient genetic diversity of germplasm resources, etc.

Active Publication Date: 2020-07-28
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

my country's taro germplasm resources are extremely rich, but there are few studies on the research and protection of genetic diversity of germplasm resources. At present, SSR technology is widely used in the research and development of molecular genetic markers. The primers are designed by tandem sequences of dozens of nucleotides composed of distributed repeat units. The development of SSR-labeled primers is firstly to obtain the sequence information on both sides of the repeat sequence from the species and design primers, and then it can be used; However, with the development of taro breeding technology, many new taro varieties

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  • SSR primer group of taro and application thereof
  • SSR primer group of taro and application thereof
  • SSR primer group of taro and application thereof

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Effect test

Embodiment 1

[0029] 1. Experimental method:

[0030] (1) Main equipment:

[0031] (1) PCR Amplifier: K960 Hangzhou Lattice Scientific Instrument Co., Ltd.

[0032] (2) Genetic analyzer: 3730XL ABI American Applied Biosystems

[0033] (3) Horizontal electrophoresis tank: JY-SPFT Beijing Junyi Dongfang Electrophoresis Equipment Co., Ltd.

[0034] (4) Horizontal electrophoresis apparatus: JY300C Beijing Junyi Dongfang Electrophoresis Equipment Co., Ltd.

[0035] (5) Gel imaging analysis system: K 8160 Beijing Kechuang Ruixin Biotechnology Co., Ltd.

[0036] (2) Main reagents

[0037] (1) Taq enzyme, dNTP: TaKaRa, Bao Bioengineering (Dalian) Co., Ltd.

[0038] (2) DL2000: TaKaRa, Bao Biological Engineering (Dalian) Co., Ltd.

[0039] (3) Primers were synthesized in General Biosystems (Anhui) Co., Ltd.

[0040] (3) SSR primer construction method:

[0041] 1. Extraction method of taro genomic DNA:

[0042] The modified CTAB method was used for DNA extraction, and about 0.5g of each samp...

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Abstract

The invention relates to the technical field of molecular markers, especially to a primer group of a taro and application thereof. According to the invention, the taro is used as a material, and the DNA of the taro is extracted and subjected to enzyme digestion, sequencing and analysis to obtain corresponding SSR primers, so more effective SSR markers can be provided for application research of genetic diversity and genetic relationship of the taro; analysis shows that the primers have the characteristic of high polymorphic percentage, and amplification effect is better when the primers are used for verification; selection of the primer group provides a valuable genetic tool for research, such as QTL analysis, genetic relationship identification, genetic diversity research and heterosis prediction, of the taro in genetic breeding, and a reference basis can be provided for reasonable selection and matching of breeding parents of the taro and innovative utilization of taro germplasm through research on the genetic relationship between pedigree parents.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to a taro SSR primer set and its application. 【Background technique】 [0002] Taro, also known as taro, taro, is the underground bulb of the Araceae plant. The shape and flesh quality vary according to the variety. It is a perennial tuber plant and is often cultivated as an annual crop. my country's taro germplasm resources are extremely rich, but there are few studies on the research and protection of genetic diversity of germplasm resources. At present, SSR technology is widely used in the research and development of molecular genetic markers. The primers are designed with tandem sequences of dozens of nucleotides composed of distributed repeat units. The development of SSR-labeled primers must first obtain the sequence information on both sides of the repeat sequence from the species and design the primers before they can be used; However, with the development of taro ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2600/156C12Q2525/151
Inventor 何芳练董伟清胡祥红
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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