Metabolic markers for liver and gall disease diagnosis
A technology for metabolic markers and liver and gallbladder diseases, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as insufficient specificity, low serum AFP content, and elevated AFP
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Embodiment 1
[0081]Example 1: Screening of diagnostic markers for hepatobiliary diseases
[0082] 1. Research object
[0083] The clinical serum and urine samples in this study came from 3 independent medical centers, and the specific sample information is shown in Table 1.
[0084] Table 1 Clinical serum and urine sample information
[0085]
[0086] 2. Ultra-high performance liquid chromatography tandem mass spectrometry analysis of target metabolites in serum and urine samples
[0087] 2.1 Information on target metabolites
[0088] Fifteen important differential metabolites were selected from the core metabolic phenotypes of liver diseases as the target metabolites for this screening. See Table 2 for details.
[0089] Table 2 Target metabolites and internal standard information
[0090]
[0091] * Labeled metabolites are used as internal standards
[0092] 2.2 Instruments and reagents
[0093] Ultra-high performance liquid chromatograph series triple quadrupole rod, the mod...
Embodiment 2
[0128] Example 2: Construction of a diagnostic model of hepatobiliary cancer and diagnostic accuracy testing using 4 serum metabolic markers
[0129] The research objects of this example are from 60 cases of liver cancer and 60 cases of cholangiocarcinoma serum samples and 60 cases of healthy control serum samples with normal physical examination from 3 independent medical centers, which are from the same source as the characteristic screening samples. The detection and analysis method of Example 2 of the present invention is the same as that of Example 1 of the present invention. With 4 serum metabolic combinations (GCDA+CDCA+LPC18-0+AA) as diagnostic features, 754 examples (340 examples of health, 220 examples of liver cancer, and 194 examples of cholangiocarcinoma) in Example 1 of the present invention were used as training sets. In Example 2, 180 samples were used as a test set to investigate the diagnostic accuracy of the screened serum diagnostic marker combination (GCDA...
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