In vitro shoot culturing method of hydrangea paniculata

A panicle hydrangea, cultivation method technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as no related reports, and achieve the effects of high reproduction rate, low browning rate, and low pollution rate

Active Publication Date: 2020-10-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is no relevant report on the research on the...

Method used

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  • In vitro shoot culturing method of hydrangea paniculata
  • In vitro shoot culturing method of hydrangea paniculata
  • In vitro shoot culturing method of hydrangea paniculata

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Embodiment Construction

[0025] 1. Material: 'Pink Fairy', one of the panicle hydrangea varieties, select the terminal buds and axillary buds of the young and tender stems of the year.

[0026] 2. Methods and steps:

[0027] 2.1 Collection of explants: The time is selected from April to June, under the conditions of fine weather and suitable humidity, and the selected variety is 'Pink Fairy'. Select spring shoots that grow vigorously, are free from diseases and insect pests, and have full axillary buds as materials. When collecting explants, it is best to take the young and tender internal branches of the year, so as to avoid unnecessary losses to the plants. Immediately after collection, put the cut part into a container filled with water to prevent dehydration and inactivation, and then process and inoculate in time.

[0028] 2.2 Material pretreatment: Trim the collected 'Pink Fairy' branches, remove the leaves and leave some petioles, cut them into small sections of about 2cm, and keep at least 1...

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Abstract

The invention discloses an in vitro shoot culturing method of hydrangea paniculata. The in vitro shoot culturing method comprises the steps of collecting explants; soaking the explants in washing powder, performing flushing with running water, performing soaking in mixed liquid of carbendazol and streptomycin, performing flushing with bacteria-free water, and then performing surface disinfection:performing soaking in 75% alcohol, performing flushing with the bacteria-free water, performing soaking in an HgCl2 solution and performing flushing with the bacteria-free water; trimming cutouts in two ends of each explant which is sterilized and disinfected, performing cutting to obtain stems with buds, and vertically inoculating the stems with buds in a starting culture medium; cutting tissue culture seedlings subjected to starting culture to obtain stems with buds, cutting off leaves which can be in contact with the culture medium, and perpendicularly inoculating the stems in a propagationculture medium; and performing propagation culture, then trimming basal parts of the tissue culture seedlings, transferring the trimmed tissue culture seedlings to a rooting culture medium, and inducing roots. The method is good in starting effects and low in browning rate and pollution rate, the browning rate is 0, the pollution rate is as low as 5%, the germination induction rate and the propagation coefficient are high, the fermination rate is 100%, the proliferation coefficient is as high as 7, the rooted seedlings are strong, the rooting rate is 100%, the average root quantity is 22, andthe average root length is 3.10cm.

Description

technical field [0001] The invention relates to plant cultivation technology, in particular to a method for cultivating isolated buds of hydrangea paniculata. Background technique [0002] Hydrangea paniculata Sieb.et Zucc is a deciduous shrub belonging to the genus Hydrangea in the saxifrage family. Branches dark reddish brown or taupe. Leaves are papery, 2-3 opposite or in whorls, ovate or elliptic, with serrated margins and hairy backs. Pane hydrangea is large and beautiful, with peculiar flower shape, up to 30-40cm long, flowering period from July to October, long flowering period and pleasant color. Native to Japan and Sichuan, China. It has been widely used and studied since it was introduced to Europe in the 18th century. It is a high-end flower, but it has not been popularized in China. It can be planted in the courtyard to make flower fences and flower mirrors, or cut off the flower bulbs and put them in vases indoors, which has high ornamental value. Not only ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈婷婷孟紫莲王红梅刘春风胡众恒
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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