In vitro shoot culturing method of hydrangea paniculata

Abstract

The invention discloses an in vitro shoot culturing method of hydrangea paniculata. The in vitro shoot culturing method comprises the steps of collecting explants; soaking the explants in washing powder, performing flushing with running water, performing soaking in mixed liquid of carbendazol and streptomycin, performing flushing with bacteria-free water, and then performing surface disinfection:performing soaking in 75% alcohol, performing flushing with the bacteria-free water, performing soaking in an HgCl2 solution and performing flushing with the bacteria-free water; trimming cutouts in two ends of each explant which is sterilized and disinfected, performing cutting to obtain stems with buds, and vertically inoculating the stems with buds in a starting culture medium; cutting tissue culture seedlings subjected to starting culture to obtain stems with buds, cutting off leaves which can be in contact with the culture medium, and perpendicularly inoculating the stems in a propagationculture medium; and performing propagation culture, then trimming basal parts of the tissue culture seedlings, transferring the trimmed tissue culture seedlings to a rooting culture medium, and inducing roots. The method is good in starting effects and low in browning rate and pollution rate, the browning rate is 0, the pollution rate is as low as 5%, the germination induction rate and the propagation coefficient are high, the fermination rate is 100%, the proliferation coefficient is as high as 7, the rooted seedlings are strong, the rooting rate is 100%, the average root quantity is 22, andthe average root length is 3.10cm.

Description

technical field [0001] The invention relates to plant cultivation technology, in particular to a method for cultivating isolated buds of hydrangea paniculata. Background technique [0002] Hydrangea paniculata Sieb.et Zucc is a deciduous shrub belonging to the genus Hydrangea in the saxifrage family. Branches dark reddish brown or taupe. Leaves are papery, 2-3 opposite or in whorls, ovate or elliptic, with serrated margins and hairy backs. Pane hydrangea is large and beautiful, with peculiar flower shape, up to 30-40cm long, flowering period from July to October, long flowering period and pleasant color. Native to Japan and Sichuan, China. It has been widely used and studied since it was introduced to Europe in the 18th century. It is a high-end flower, but it has not been popularized in China. It can be planted in the courtyard to make flower fences and flower mirrors, or cut off the flower bulbs and put them in vases indoors, which has high ornamental value. Not only ...

AI Technical Summary

Problems solved by technology

[0004] There is no relevant report on the research on the in vitro bud culture technology of Pane hydrangea 'Pink Fairy'

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