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Application of cryopreservation solution containing peptide compound in stem cell cryopreservation

A cryopreservation solution and cryopreservation technology, applied in the field of biomedical materials, can solve the problems of not having ice crystal growth, not being able to effectively control ice crystal growth, damaging cells, etc.

Active Publication Date: 2020-10-20
PEKING UNIV THIRD HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing cryopreservation reagents cannot effectively control the growth of ice crystals during rewarming, which can damage cells
The high concentration (≥15%) toxic organic solvents used in the current vitrification method, such as: DMSO, lead to toxic side effects of cryopreservation reagents on cells, seriously affecting the survival rate and even the safety of cryopreservation objects after recovery sexual and functional expression
In summary, the cryopreservation reagents currently used do not have the ability to effectively control the growth of ice crystals during the rewarming process, and there is also the problem of poor reagent safety.

Method used

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  • Application of cryopreservation solution containing peptide compound in stem cell cryopreservation
  • Application of cryopreservation solution containing peptide compound in stem cell cryopreservation
  • Application of cryopreservation solution containing peptide compound in stem cell cryopreservation

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preparation example Construction

[0084] The preparation method of the present invention will be further described in detail in conjunction with specific examples below. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0085] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the reagents and materials used in the following examples, unless otherwise specified, can be obtained from commercial sources.

Embodiment 1

[0086] Synthesis of embodiment 1 formula (1) compound

[0087] (1) Put 2-chlorotrityl chloride resin (2-Chlorotrityl Chloride Resin) into the reaction tube, add DCM (20mL g -1 ), shaking for 30 minutes. The sand core was filtered to remove the solvent, and a three-fold molar excess of Fmoc-L-Pro-OH was added, followed by an eight-fold molar excess of DIEA, and finally DMF was added to dissolve, and shaken for 30 minutes. Methanol capping for 30 minutes.

[0088] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10mL g -1 ), after 5 minutes the solvent was removed, and a 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it three times with ethanol, add ninhydrin reagent, heat at 105-110°C for 5 minutes, if it turns dark blue, it is a positive reaction.

[0089] (3) The product obtained by the above reaction was washed with DMF (15mL g -1 , twice), methanol (15mL g -1 , twice) and...

Embodiment 2

[0095] Synthesis of embodiment 2 formula (2) compound

[0096] (1) Put 2-chlorotrityl chloride resin into the reaction tube, add DCM (20mL g -1 ), shaking for 30 minutes. The sand core was filtered to remove the solvent, and a three-fold molar excess of Fmoc-L-Thr(tBu)-OH was added, followed by an eight-fold molar excess of DIEA, and finally DMF was added to dissolve, and shaken for 30 minutes. Methanol capping for 30 minutes.

[0097] (2) Remove the solvent DMF, add 20% piperidine / DMF solution (10mL g -1 ), after 5 minutes the solvent was removed, and a 20% piperidine / DMF solution (10 mL g -1 ), the piperidine solution was removed after 15 minutes. Take a small amount of resin, wash it three times with ethanol, add ninhydrin reagent, heat at 105-110°C for 5 minutes, if it turns dark blue, it is a positive reaction.

[0098] (3) The product obtained by the above reaction was washed with DMF (15mL g -1 , twice), methanol (15mL g -1 , twice) and DMF (15mL g -1 , twice) a...

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Abstract

The invention discloses an application of a cryopreservation solution containing a peptide compound in stem cell cryopreservation. The peptide compound is composed of ice-philic amino acid and a hydrophilic group, has good capabilities of inhibiting ice crystal growth and modifying ice crystal morphology in an aqueous solution, has no thermal hysteresis, has good biocompatibility, and is an idealice control material. The cryopreservation solution containing the peptide compound contains the peptide compound, polyol, water-soluble sugar and other components, and can maintain a good survival rate when used for stem cell cryopreservation.

Description

[0001] This application claims the priority of the previous application filed with the State Intellectual Property Office of China on April 9, 2019 with the patent application number 201910281986.7 and the title of the invention "A Peptide Compound and a Cryopreservation Solution Containing the Compound". The entirety of this prior application is incorporated by reference into the present application. technical field [0002] The invention belongs to the technical field of biomedical materials, and in particular relates to the application of a cryopreservation solution containing peptide compounds in stem cell cryopreservation. Background technique [0003] Cryopreservation refers to the preservation of biological materials at ultra-low temperature, which slows down or stops the metabolism and division of cells, and can continue to develop once the normal physiological temperature is restored. Since the technology came out, it has become one of the indispensable research met...

Claims

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Application Information

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IPC IPC(8): C07K5/062C07K5/068C07K5/078C07K5/083A01N1/02
CPCC07K5/0606C07K5/06095C07K5/06165C07K5/0806C07K5/081A01N1/0221
Inventor 乔杰严杰闫丽盈李蓉王健君金晟琳
Owner PEKING UNIV THIRD HOSPITAL
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