Tulasnella fungus and application thereof in promoting germination of paphiopedilum spicerianum seeds to form seedlings

Abstract

The invention relates to a tulasnella fungus and an application thereof in promoting germination of paphiopedilum spicerianum seeds to form seedlings. The fungus is a tulasnella strain which is Tulasnella sp. GYBQ01, and the preservation number of the fungus in China Center for Type Culture Collection is CCTCC NO: M 2020129. The invention provides morphology and physiological and biochemical characteristics of the strain. Morphology, physiology, biochemistry and nucleotide sequence analysis results show that Tulasnella sp. GYBQ01 belongs to a tulasnellaceae tulasnella fungus, can be used for promoting germination of the paphiopedilum spicerianum seeds to form seedlings, and has higher specificity, high-quality symbiotic seedlings can be obtained through inoculating the paphiopedilum spicerianum seeds with the strain, efficient seed and seedling breeding of symbiotic germination can be realized, and the Tulasnella sp. GYBQ01 can be used for seedling culture in nurseries or field fungusseed dressing and direct sowing. Compared with tissue culture, the tulasnella fungus has the advantages of being low in cost, high in field cultivation survival rate, free of tissue degeneration and the like, and lays a foundation for carrying out bionic cultivation, species regression and population reconstruction of the paphiopedilum spicerianum.

Description

technical field [0001] The invention relates to a fungus of the genus Glycophaga and the application thereof for promoting the germination of Paphiopedilum papillosa seeds to form seedlings, and belongs to the field of applied microorganisms. Background technique [0002] Paphiopedilum alba ( Paphiopedilum spicerianum ), was first found in Assam in northern India, and later in northeastern India along the border with Myanmar. The sepals in the flowers are large and white, like a white flag, the upper part is curved forward into an arch, and the pseudostamens are ear-shaped and heart-shaped, which has high ornamental and breeding value. In 2006, in Pu'er City, Yunnan Province, my country, the wild distribution of P. albicans was discovered for the first time, and it is also the only confirmed wild distribution point in my country. There are only more than 20 plants in the original population. This species grows on the steep bank of a small river ditch at the entrance of an ...

AI Technical Summary

Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome. , meanwhile, whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi at the seed germination stage is still uncertain