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Leukocyte counting and typing instrument and leukocyte counting and typing method

A technology of white blood cell counting and spectroscopic film, which is applied in the direction of instruments, analytical materials, scientific instruments, etc., can solve the problems of incomplete red blood cell lysis, short detection time window, white blood cell dissolution, etc., achieve short staining time, easy storage and transportation, Use simple effects

Pending Publication Date: 2020-11-10
苏州中科苏净生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hemolysis counting method adopted by HemoCue has the following disadvantages: first, the method uses the transmission absorption method, which is easily interfered by impurities; second, the detection time window is short, and the detection must be strictly controlled within 2-10 minutes, and the red blood cell lysis is too short Incomplete, some white blood cells will be dissolved if the time is too long; the third is to distinguish the white blood cell type through geometric features, the accuracy is low

Method used

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  • Leukocyte counting and typing instrument and leukocyte counting and typing method
  • Leukocyte counting and typing instrument and leukocyte counting and typing method
  • Leukocyte counting and typing instrument and leukocyte counting and typing method

Examples

Experimental program
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Effect test

Embodiment 1

[0081] Figure 11A-Figure 11C It is an example of the structure of the detection card 4 of the present invention, which is designed in the form of a slit microfluidic chip. Figure 11A-Figure 11C In the first shown embodiment, the detection card includes a support substrate 21 and a sampling detection cavity 22 arranged on the support substrate 21, wherein the support substrate 21 is the hand-held part of the detection card, which is designed to be suitable for hand-held shape, such as In the first embodiment, the handle is rectangular, and the front end is extended with a sampling and detection chamber 22 with arc-shaped edges; The sampling detection chamber 22 is a semi-open cavity formed by two parallel chamber side walls 24 with a certain gap, including a sampling port 27, a sampling notch 29, a detection area 25, and a flow diversion connecting the sampling port 27 and the detection area 25 Tank area 26, wherein:

[0082] The sampling port 27 is located at the opening o...

Embodiment 2

[0095] The detection card of this embodiment is the same as that of Embodiment 1, and the staining agent is acridine orange.

[0096] The human-computer interaction module II mainly includes the shell 14 of the instrument, the detection card cabin door, the display screen 11 and the function keys, among which the shell 14 is used to assemble the internal components of the instrument and install the display screen, function keys, etc. Put into detection card 4, display screen 11 and function key complete the input of user instruction and the output of detection result; to start the detection program, the return key is used to return to the main page.

[0097] The main control unit 8 is responsible for the control of the working process of the instrument, and is electrically connected with the function keys and the display screen on the human-computer interaction module, and is electrically connected with the photoelectric detection module. After the human-computer interaction m...

Embodiment 3

[0114] This embodiment is only different from the second embodiment in the photoelectric detection module, such as figure 2 As shown, it is composed of a light source 1, a collimating lens 2, a filter 3, a beam splitter 9, a fluorescence filter 5, an imaging lens 6, an image sensor 7, a control unit 8, and the like. The light source 1, the collimating lens 2, the optical filter 3 and the beam splitter 9 are sequentially arranged on a horizontal straight line along the propagation direction of the light, and the beam splitter 9, the fluorescence filter 5, the imaging lens 6 and the image sensor 7 are successively arranged on the On another vertical straight line, the horizontal straight line where the light source 1, the collimating lens 2 and the optical filter 3 are located is perpendicular to the vertical straight line where the fluorescent filter 5, the imaging lens 6, etc. are located, and the focus of the two straight lines (i.e. vertical foot) is the position of the bea...

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Abstract

The invention discloses a leukocyte counting and typing instrument and a leukocyte counting and typing method. The leukocyte counting and typing instrument comprises a man-machine interaction module,a photoelectric detection module, a main control unit and a detection card; the detection card is arranged in the photoelectric detection module, and the main control unit is electrically connected with the man-machine interaction module and the photoelectric detection module. The leukocyte counting and typing instrument does not need hemolysis or pH value adjustment, and is short in staining time, simple to operate and accurate in leukocyte classification. The leukocyte counting and typing method provided by the invention not only can be used for typing and counting leukocytes, but also can be used for imaging and counting neutrophils, lymphocytes and mononuclear cells.

Description

technical field [0001] The invention relates to the technical field of blood analysis and detection, in particular to an instrument for counting and typing white blood cells and a method for counting and typing white blood cells. Background technique [0002] There are three types of blood cells in the human body: white blood cells, red blood cells, and platelets. White blood cells mainly include granulocytes, lymphocytes and monocytes. Among them, granulocytes account for 50-70% of white blood cells, lymphocytes account for 20-40%, and monocytes account for 1-7%. Under normal circumstances, the total number of white blood cells in the human body and the percentages of various types of white blood cells are relatively stable, but inflammation or other diseases will cause changes in their values. In other words, the occurrence of inflammation or other diseases in the body can cause changes in the total number of white blood cells and the percentages of various types of whit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64
CPCG01N15/1434G01N15/1484G01N21/6428G01N21/6486G01N2015/1006G01N2015/144G01N2015/1486
Inventor 李悦琴
Owner 苏州中科苏净生物技术有限公司
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