Air pressure acceleration type nucleic acid imprinting membrane transfer device and membrane transfer method thereof

Abstract

The invention discloses an air pressure acceleration type nucleic acid imprinting membrane transfer device and a membrane transfer method thereof. Membrane transferring of an existing membrane transferring device usually needs 16-18 h, and the process is long. The device comprises a bottom plate, an inflation bin, a middle membrane transfer platform, an air inlet pipe and an air source. The inflation bin is installed on the bottom plate. An inner cavity of the inflation bin is connected with an air source through one or more air inlet pipes. The bottom of the middle membrane transfer platformis located in the inflation bin, and the top of the middle membrane transfer platform extends out of the top of the inflation bin. And the air source comprises a shunt pipe, a pressure regulator and an air pump. An air inlet of the pressure regulator is communicated with an air outlet of the air pump, and an air outlet is connected to an inner cavity of the inflation bin through one or more air inlet pipes. The buffer solution is added in an atmospheric pressure increasing manner, so that the film transfer membrane is greatly shortened from original 16-18 hours to 1.5 hours; in addition, the inflation bin further plays a role in reinforcing the middle film transfer platform, so that the structure of the membrane transfer device is more stable, and the membrane is not prone to deviating.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid imprinting transfer membrane, and in particular relates to an air pressure accelerated nucleic acid imprinting transfer device and a membrane transfer method. Background technique [0002] Southern Blot (i.e., southern blot hybridization) is a basic technique for studying DNA maps. Under certain conditions, two nucleic acid single strands with certain homology can specifically hybridize according to the principle of base complementarity to form double strands. Generally, agarose gel electrophoresis is used to separate the DNA fragments digested by restriction endonucleases, the DNA on the gel is denatured and the single-stranded DNA fragments are transferred to nylon membranes or other solid supports in situ, and dry-baked or It is fixed by ultraviolet radiation, and then hybridized with the labeled probe of the corresponding structure, and the content of specific DNA molecules is detected af...

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Problems solved by technology

The method is simple to operate, but there is no fixed instrument and equipment; a water-absorbing filter paper layer with a height of 12cm needs to be laid on the nylon membrane, and a weight of 400g-700g is pressed on the water-absorbing filter paper layer, and the water-absorbing filter paper layer needs to be replaced every hour during the membrane transfer. It is cumbersome, and it is easy to cause the nylon membrane to shift during the replacement process; the membrane transfer usually takes 16-18 hours, and the process is long; the water-absorbing filter paper layer has no fixed support structure, and the stability is poor, prone to rollover, and prone to left and right The experiment failed due to the uneven distribution of DNA on the membrane caused by different water absorption speeds on both sides and unbalanced weight pressure.

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