Fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application

A technology of oral epithelial cells, Fusobacterium nucleatum, applied in the field of biomedicine

Pending Publication Date: 2020-11-24
HOSPITAL OF STOMATOLOGY CHINA MEDICAL UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the lncRNA changes involved in the malignant transformation of oral

Method used

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  • Fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application
  • Fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application
  • Fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application

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Embodiment

[0057] The human immortalized oral epithelial cells HIOECs and F.nucleatum ATCC25586 bacteria used in the implementation of the present invention are all preserved by the Central Laboratory of the Stomatological Hospital Affiliated to China Medical University, and belong to the materials available to the public.

[0058] The present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments, which are intended to explain rather than limit the present invention.

[0059] A specific embodiment of the present invention provides a research method for differentially expressing mRNA and lncRNA during the malignant transformation of human oral epithelial cells infected with Fusobacterium nucleatum, and screening tumor-related core genes, including the following steps:

[0060] 1. Preparation of common reagents

[0061] 1.1 HIOECs cell culture reagents: modified keratinocyte serum-free medium Defined keratinocyte-SFM (10744...

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Abstract

The invention discloses a fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application. The biomarker comprises 15 lncRNAs (long non-coding RNAs) such as AC118757.1,AL162727.1 and AC090844.3. During screening, the human immortalized oral epithelial cells HIOECs are resuscitated and cultured; a fusobacterium nucleatum model strain ATCC25586 is resuscitated; an in-vitro model of the fusobacterium nucleatum infected HIOECs cells is constructed; total RNA of HIOECs infected by fusobacterium nucleatum and uninfected HIOECs cells are extracted respectively to carryout high-throughput sequencing on lncRNA and mRNA transcriptomes, differentially expressed mRNA and lncRNA are screened to further determine a core gene; and the lncRNA participating in differentialexpression of a core gene is screened by constructing an lncRNA-core gene co-expression network. An analysis result can lay a good foundation for further research of fusobacterium nucleatum in the process of affecting malignant transformation and tumors.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a biological marker of oral epithelial cell tumor associated with Fusobacterium nucleatum and its screening application. Background technique: [0002] A large number of epidemiological studies have found that about 20% of malignant tumors are related to chronic infectious factors. Bacteria, as potential carcinogenic factors, can participate in the regulation of host cell function and gene expression. Oral cancer is one of the most common malignant tumors in the head and neck, among which oral squamous cell carcinoma (OSCC) accounts for about 90%. Fusobacterium nucleatum is an important periodontal pathogen and one of the resident intestinal flora. The study found that Fusobacterium nucleatum had a high detection rate in OSCC tissues, suggesting that Fusobacterium nucleatum may be associated with oral tumors. In vitro cell experiments have also found that F. nu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/178C12Q2600/158
Inventor 潘亚萍张树伟李琛李玉超张智颖
Owner HOSPITAL OF STOMATOLOGY CHINA MEDICAL UNIV
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