Preparation method of exosome-like structural lipid microcapsule

Abstract

The invention discloses a preparation method of an exosome-like structural lipid microcapsule. The preparation method comprises the following steps of: A, culturing PBMC cells by using a culture medium to obtain in-vitro mature dendritic cells; B, adding Proteinase K into the in-vitro mature dendritic cells to perform pre-treatment; C, adding an exosome extraction reagent, the volume of which is 0.3-1.2 times of that of the in-vitro mature dendritic cells, into the pre-treated in-vitro mature dendritic cells, centrifuging for 30-50 min, and collecting supernatant with the centrifugal force of3000-5000 g; and D, centrifuging the supernatant for 100-150 min, and collecting the supernatant again. The lipid microcapsule prepared by the method is small in volume and easy in in-vivo circulation; the lipid microcapsule can reach damaged tissues easily; meanwhile, the absorption speed and the absorption amount of the lipid microcapsule by gastrointestinal mucous membranes of organisms are also increased; the lipid microcapsule is good in in-vivo absorption; therefore, the effects of relatively high bioavailability and rapid drug effect exertion are achieved; and furthermore, the lipid microcapsule is mild in preparation condition, simple, controllable, suitable for large-scale production and low in preparation cost, and has price advantage.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for preparing lipid microcapsules with an exosome-like structure. Background technique [0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Today, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. In 1983, exosomes were first produced in sheep reticulocytes It was discovered that in 1987 Johns tone named it "exosome". A variety of cells can secrete exosomes under normal and pathological conditions, which are mainly derived from the multivesicular bodies formed by the invagination of lysosomal particles in cells. It is released into the extracellular matrix after the fusion of the outer membrane of the multivesicle and the cell membrane. The microcapsule on the right side of the microcapsule refers to a solid or liquid drug (core or fill material) wrapped by a shell material. Drug store-type microc...

AI Technical Summary

Problems solved by technology

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Today, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. In 1983, exosomes were first produced in sheep reticulocytes It was discovered that in 1987 Johns tone named it "exosome". A variety of cells can secrete exosomes under normal and pathological conditions, which are mainly derived from the multivesicular bodies formed by the invagination of lysosomal particles in cells. It is released into the extracellular matrix after the fusion of the outer membrane of the multivesicle and the cell membrane. The microcapsule on the right side of the microcapsule refers to a solid or liquid drug (core or fill material) wrapped by a shell material. Drug store-type microcapsules, the drugs contained in the microcapsules can be released in specific parts and media, and have different drug release characteristics such as sustained release, controlled release or targeted release, but the existing lipid microcapsules are relatively small in volume. Large, not easy to be absorbed by the body's gastrointestinal mucosa, and the preparation requirements are high and the process is complicated, which increases its preparation cost. Therefore, we propose a method for the preparation of lipid microcapsules with an exosome-like structure