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Method for increasing alkaloid content of subprostrate sophora tissue culture seedlings by using calcium ions

A technology of calcium ion and tissue culture seedlings, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of low alkaloid content, affecting the utilization rate of mountain bean roots, etc., and achieve the effect of improving the drying rate

Inactive Publication Date: 2021-01-01
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The alkaloids in the root of the mountain bean are the main active ingredients and the material basis of its medicinal effect. However, the content of alkaloids in the tissue cultured seedlings of the root of the mountain is low at present, which affects the utilization rate of the root of the mountain.

Method used

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  • Method for increasing alkaloid content of subprostrate sophora tissue culture seedlings by using calcium ions
  • Method for increasing alkaloid content of subprostrate sophora tissue culture seedlings by using calcium ions
  • Method for increasing alkaloid content of subprostrate sophora tissue culture seedlings by using calcium ions

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Effect test

Embodiment 1

[0018] The method for utilizing calcium ions to increase the alkaloid content of the root tissue cultured seedlings of the mountain bean root comprises the following steps:

[0019] Step 1, adding exogenous calcium ions, naphthalene acetic acid, and indole acetic acid to the 1 / 2MS medium to obtain the first medium, wherein the concentration of exogenous calcium ions is 0.5 times the concentration of calcium ions in the 1 / 2MS medium ;

[0020] Step 2, inoculating the aseptic seedlings of the root of the mountain bean in the first culture medium to obtain the tissue-cultured seedling of the root of the root of the mountain root.

[0021] In step 2, the cultivation conditions are as follows: the temperature is 22° C., the light time is 12 h / d, the light intensity is 2500 Lux, and the culture time is 40 days.

[0022] In Step 1, the exogenous calcium ions are derived from calcium chloride.

[0023] In step one, the first culture medium also includes sucrose, agar powder, and act...

Embodiment 2

[0026] The method for utilizing calcium ions to increase the alkaloid content of the root tissue cultured seedlings of the mountain bean root comprises the following steps:

[0027] Step 1, adding exogenous calcium ions, naphthalene acetic acid, and indole acetic acid to 1 / 2MS medium to obtain the first medium, wherein the concentration of exogenous calcium ions is 4 times the concentration of calcium ions in 1 / 2MS medium ;

[0028] Step 2, inoculating the aseptic seedlings of the root of the mountain bean in the first culture medium to obtain the tissue-cultured seedling of the root of the root of the mountain root.

[0029] In step 2, the cultivation conditions are as follows: the temperature is 28°C, the light time is 12h / d, the light intensity is 3000Lux, and the culture time is 50d.

[0030] In Step 1, the exogenous calcium ions are derived from calcium chloride.

[0031] In step one, the first culture medium also includes sucrose, agar powder, and activated carbon.

...

Embodiment 3

[0034] The method for utilizing calcium ions to increase the alkaloid content of the root tissue cultured seedlings of the mountain bean root comprises the following steps:

[0035] Step 1, adding exogenous calcium ions, naphthalene acetic acid, and indole acetic acid to the 1 / 2MS medium to obtain the first medium, wherein the concentration of exogenous calcium ions is 0.5 times the concentration of calcium ions in the 1 / 2MS medium ;

[0036] Step 2, inoculating the aseptic seedlings of the root of the mountain bean in the first culture medium to obtain the tissue-cultured seedling of the root of the root of the mountain root.

[0037] In step 2, the cultivation conditions are as follows: the temperature is 25°C, the light time is 12h / d, the light intensity is 2750Lux, and the culture time is 40-50d.

[0038] In Step 1, the exogenous calcium ions are derived from calcium chloride.

[0039] In step one, the first culture medium also includes sucrose, agar powder, and activate...

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Abstract

The invention discloses a method for increasing the alkaloid content of subprostrate sophora tissue culture seedlings by using calcium ions. The method comprises the following steps that firstly, exogenous calcium ions, naphthylacetic acid and indoleacetic acid are added into a 1 / 2MS culture medium to obtain a first culture medium, and the concentration of the exogenous calcium ions is 0.5-4 timesof the concentration of the calcium ions in the 1 / 2MS culture medium; and secondly, subprostrate sophora aseptic seedlings are inoculated into the first culture medium to be cultured to obtain subprostrate sophora tissue culture seedlings. In the second step, according to the culture conditions, the temperature is 22-28 DEG C, the illumination time is 12 h / d, the illumination intensity is 2500-3000 Lux, and the culture time is 40-50 d. In the first step, the exogenous calcium ions are derived from calcium chloride. In the first step, the first culture medium further comprises saccharose, agarpowder and activated carbon. By introducing the exogenous calcium ions into the culture medium, the content of alkaloid in the subprostrate sophora tissue culture seedlings can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of mountain bean root cultivation. More specifically, the present invention relates to a method for increasing the content of alkaloids in the tissue cultured seedlings of bean root by utilizing calcium ions. Background technique [0002] Shandougen is a famous authentic medicinal material and a commonly used bulk traditional Chinese medicine in Guangxi. It is also called Guanggendou. It is the dried root and rhizome of the leguminous plant Sophora tonkinensis Gagnep. It is the main raw material of nearly 30 kinds of Chinese patent medicines for treating hepatitis, sore throat, anti-tumor, hemorrhoids, acne, and skin diseases. According to the standard of "Chinese Pharmacopoeia" (2015 edition), the total amount of matrine and oxymatrine contained in mountain bean root should not be less than 0.70%. The alkaloids are the main active ingredients and the material basis of its medicinal effect. However, the al...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁莹韦坤华李林轩秦双双林杨韦范缪剑华韦桂丽陈娟
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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