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A small animal autonomous social behavior testing device and experimental method

A technology of testing device and experimental method, which is applied to animal houses, counting mechanisms/objects, instruments, etc., to achieve the effects of simple splicing, easy cleaning, and convenient assembly.

Active Publication Date: 2022-07-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to subdivide the psychological changes in the whole social process, and then subdivide the dynamic changes of EEG, and then further explain the dynamic changes of EEG, is currently a major problem in this field, and there is no related experimental device yet or experimental paradigms applied to the study of autonomous social behavior in animals

Method used

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  • A small animal autonomous social behavior testing device and experimental method
  • A small animal autonomous social behavior testing device and experimental method
  • A small animal autonomous social behavior testing device and experimental method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] An experimental method for applying the above-mentioned small animal autonomous social behavior testing device, comprising the following steps:

[0046] Step 1: Purchase 21-day-old SD rats (SD rat weaning age) from an experimental animal company that has the license to produce and sell experimental animals, and randomly pair them in a cage of 4, mark each animal, weigh and record the body weight , After the animals are purchased, they are raised in a constant temperature and humidity environment with reversed day and night to ensure adequate supply of feed and drinking water until 70 days of age (the adult SD rat);

[0047] In addition, day and night reversal refers to the environment in which animals are raised is completely dark during the day and lights up at night, because mice and mice are nocturnal creatures, it is more reasonable to do experiments at night;

[0048] Step 2: Put rat A into test chamber 1, and place one rat X and rat Y in the same sex as rat A and ...

Embodiment 2

[0059] The basic steps are the same as in Example 1. The difference from Example 1 is that after the completion of Step 7, a craniotomy is performed on rat A, and electrodes are embedded in the test brain area (such as the prefrontal lobe) of rat A. Let rat A rest, and after 7 days, repeat steps 2-7 in Example 1, the difference is that while the steps 2-7 are carried out, the discharge of rat A is recorded synchronously, and the discharge of rat A is synchronously recorded. Physiological discharges were investigated to explore the social behavior of the test animals.

Embodiment 3

[0061] The basic steps are the same as in Example 1. Steps 1-4 and 6-7 are the same (in step 7, the corresponding data analysis of the second stage of the experiment is removed). In step 5, the situation of the two storage tubes is the same as that in step 4. Adjust the gate control circuit 10 to extend the descending time of the lift gate 6, that is, rat A presses the micro switch for a period of time after the lift gate 6 is closed (this time can be adjusted, such as one minute), the lift gate cannot be opened; The time from when A insisted on pressing the microswitch 8 to give up when the door could not be opened, and the number of times that rat A pressed the microswitch 8 corresponding to the direction of rat B during the period when the door could not be opened, to determine the degree of desire of the test animals for social activities. The longer the time between pressing the microswitch 8 and giving up during the period of being unable to open the door, the more eager ...

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Abstract

A small animal autonomous social behavior testing device, comprising a test chamber, two outer walls of the test chamber are respectively provided with a pre-material pipe and a rear-mounted material pipe, and both the front-end material pipe and the rear material pipe are separated from the interior of the test chamber by a wire mesh. , The side wall of the test chamber is provided with a guide rail slot, a lifting door and a lifting mechanism that can drive the lifting door to rise and fall. The lifting mechanism includes a motor. , the micro switch is electrically connected to the lifting mechanism, the micro switch is electrically connected to the counter, the micro switch and the motor are electrically connected to the gate control circuit, and the gate control circuit includes a delay disconnection relay, a motor forward and reverse controller, a delay time A conduction relay, a sliding rheostat, and an infrared camera are also provided; and an experimental method for applying the device is provided. The invention can more accurately reflect the social initiative of animals, obtain more stable and reliable experimental results, and analyze the electrophysiological results more easily. , and the experimental device is easy to assemble and disassemble.

Description

technical field [0001] The invention relates to the field of biological experimental devices, in particular to a small animal autonomous social behavior testing device and an experimental method using the device. Background technique [0002] Social interaction is a social activity that must be carried out by social animals, including humans, on a daily basis. Many experimental results show that good social interaction has a positive effect on humans or animals, while short-term social isolation can cause anxiety in humans or animals, and long-term social isolation can greatly increase the risk of mental illness. Therefore, it is of great significance to the social research, especially the social research on the subjective will. [0003] The most representative experiment in animal socialization is the three-box socialization experiment. Although this experimental paradigm can be used to analyze the social behavior of animals, the data analyzed are the time the animal stay...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K29/00G06M1/08G06M1/28A01K1/03A01K1/035
CPCA01K29/005G06M1/083G06M1/28A01K1/031A01K1/035A01K2267/03
Inventor 金磊沈昊伟余志鹏金彬杰
Owner NINGBO UNIV
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