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A kind of modeling method of mouse model of chronic contact dermatitis

A contact dermatitis, mouse model technology, applied in the field of biomedicine, can solve problems such as inappropriateness, and achieve the effect of a good screening platform

Active Publication Date: 2021-03-12
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, it is inappropriate to use any of the above single models

Method used

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  • A kind of modeling method of mouse model of chronic contact dermatitis
  • A kind of modeling method of mouse model of chronic contact dermatitis
  • A kind of modeling method of mouse model of chronic contact dermatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Fifteen 7-week-old female Balb / c mice were selected. Except for the normal control group (3 mice), the other 12 mice received 30 μL of 1.3% oxazolone in acetone-olive oil solution on the front and back of the right ear as the first trial. For sensitization challenge, the day was designated as day 0. Seven days later, the right ear of all mice received again 30 μL of 0.8% oxazolone in acetone-olive oil solution as a second sensitization challenge. Thereafter, 20 μL of 0.3-0.5% mass fraction of oxazolone in acetone-olive oil solution was used for sensitization challenge every 2-3 days. Specifically, 0.5%, 0.5%, 0.5%, and 0.3%. On the 17th day, 12 mice with chronic contact dermatitis were obtained.

Embodiment 2

[0034]Four 6-week-old female Balb / c mice were selected to receive 30 μL of 1.2% oxazolone in acetone and olive oil solution on the front and back of the right ear as the first sensitization challenge, and this day was designated as day 0. Seven days later, the right ear of all mice received again 30 μL of 0.9% oxazolone in acetone-olive oil solution as a second sensitization challenge. Thereafter, 20 μL of 0.4-0.6% mass fraction of oxazolone in acetone-olive oil solution was used for sensitization challenge every 2-3 days. Specifically, 0.6% and 0.4% , 0.4%. Four mice with chronic contact dermatitis were obtained.

Embodiment 3

[0035] Example 3 Mouse Model Verification

[0036] Three days after the last sensitization challenge in Example 1, that is, on the 17th day, the 12 chronic contact dermatitis mice obtained were grouped according to the thickness of the right ear, and 9 mice with similar thickness of the right ear were selected and divided into three groups ( 3 animals in each group, the average thickness of the right ear is basically the same), two groups of them began to administer treatment on the same day, and one group used the positive control drug betamethasone, and the administration method was 0.03mg / ear topical administration, which was dissolved in In acetone, smear on the front and back of the right ear of the mice in the positive drug group; the other group uses dexamethasone, and the administration method is 2 mg / kg orally. Because contact dermatitis is currently being treated clinically, topical steroids are mainly used for symptom control, so in this example, the common topical ...

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Abstract

The invention provides a method for making a chronic contact dermatitis mouse model in combination with the onset characteristics and rules of the chronic contact dermatitis. This method uses oxazolone to repeatedly sensitize and challenge animals, which can not only play the role of chemical stimulation, but also simulate the whole process of patients being repeatedly exposed to allergens, finally being sensitized and leading to chronic contact dermatitis. The pathological changes of the diseased part of the obtained animal model and the patient's skin injury area are highly consistent, indicating that this model can be used to simulate the chronic contact dermatitis model of clinical patients, and provide a better screening platform for the treatment of chronic contact dermatitis.

Description

technical field [0001] The application belongs to the field of biomedicine, and in particular relates to a method for establishing an animal model of chronic contact dermatitis in mice induced by oxazolone. Background technique [0002] Contact dermatitis is a clinically very common disease, and its prevalence in the population is about 1.7%-6.3%. Although in most people, the symptoms of atopic dermatitis are not particularly serious, nor are they life-threatening. However, the repeated itching of the diseased part, and the ulceration and further aggravating symptoms caused by scratching often cause the patient to suffer very much. In general, it is not easy for patients to identify specific allergens and exclude them from their lives. Therefore, the patient's contact dermatitis is often protracted, which has a great impact on the quality of life of the patient. In order to alleviate the pain of patients, the development of related drugs has been optimized and in progress....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/20A01K2227/105A01K2267/03
Inventor 杨楠付云峰刘新陈洁罗艳段继峰
Owner 澎立生物医药技术(上海)股份有限公司
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