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A preparation method of an animal model of bronchial allergic cough

An allergic cough and animal model technology, applied in the field of biomedicine, can solve the problem of not being able to reflect the complete characteristics of allergic cough

Active Publication Date: 2021-09-21
澎立生物医药技术(上海)股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, neither of the above two models can reflect the complete characteristics of allergic cough

Method used

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  • A preparation method of an animal model of bronchial allergic cough
  • A preparation method of an animal model of bronchial allergic cough

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Experimental program
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Embodiment 1

[0029] The screening of embodiment 1 model guinea pigs

[0030] Twenty 5-week-old female Hartley guinea pigs were selected for cough sensitivity test. All the guinea pigs to be used were first stimulated with citric acid and their cough times were detected. Guinea pigs with more than 25 or less than 15 coughs were excluded. The specific operations are as follows:

[0031]Each guinea pig was placed in a body plethysmography tank, and an air flow at a flow rate of 2 L / min was provided through a nebulizer and a deflector, and allowed to adapt to the new environment for 10 minutes. Ten minutes later, each animal was exposed for 10 minutes to 0.4M citric acid aerosol via a nebulized drug delivery system at a fluid consumption rate of 0.5 mL / min. Continue to observe for 10 minutes after the nebulization ends, and the total observation time is 20 minutes. During these 20 minutes, the guinea pig cough waveform will be sent to the MacLab / 16sp through the preamplifier (MAX2, Buxco)....

Embodiment 2

[0033] Preparation and verification of embodiment 2 animal model

[0034] From the 16 up-to-standard guinea pigs screened in embodiment 1, select 12 cough times that are relatively close, and after 2 weeks of recovery, proceed as follows (divided into 4 groups, 3 in each group, the average cough times of each group of guinea pigs is shown in figure 1 Before grouping): before entering the plethysmography tank, the vehicle control (group G1) and positive drugs: dexamethasone (group G2), methoxyphenamine (group G3) or codeine (group G4) were given 1 hour in advance. One hour after drug administration or vehicle administration, each guinea pig was again placed in a body plethysmography tank, and an air flow at a flow rate of 2L / min was provided through a nebulizer and a deflector, and allowed to adapt to the new environment for 10 minutes . After 10 minutes, each guinea pig was exposed to an aerosol of 0.4M citric acid + 0.1 mM histamine for 10 minutes at a fluid consumption rate...

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Abstract

The present invention relates to an animal model of histamine and citric acid-induced bronchial allergic cough. Combining with the onset characteristics and rules of bronchial allergic cough, an animal model of histamine sensitization and citric acid-induced cough is designed, and its therapeutic window is relatively large. , Traditional antiallergic drugs and antitussive drugs can play a good therapeutic effect, which is consistent with the clinical use of relevant patients. Therefore, this animal model can be used for the evaluation and screening of related drugs.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing an animal model of bronchial allergic cough induced by histamine and citric acid. Background technique [0002] Bronchial allergic cough, also known as allergic bronchitis, is a very common clinical disease. The incidence rate of this disease is similar to that of bronchial asthma, but it is more affected by air pollution, and its incidence rate can reach more than twice that of bronchial asthma in heavily polluted areas. Most of its patients are children under 7 years old, or people over 50 years old, and more than 30% of children's dry cough is related to allergic cough. Usually patients themselves may have a clear history of allergic diseases: including eczema, allergic rhinitis or allergic dermatitis and so on. However, bronchial allergic cough has certain similarities with bronchial allergic asthma, and specific inflammatory reactions of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A01K2227/105A01K2267/03A61K49/0008
Inventor 刘新陈洁袁媛杨楠段继峰
Owner 澎立生物医药技术(上海)股份有限公司
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