Tissue culture propagation method of cinnamomum japonicum

A technique of Tianzhu cinnamon and tissue culture, which can be used in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve problems such as few reports.

Inactive Publication Date: 2021-02-05
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the tissue culture of Cinnamon plants

Method used

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  • Tissue culture propagation method of cinnamomum japonicum
  • Tissue culture propagation method of cinnamomum japonicum
  • Tissue culture propagation method of cinnamomum japonicum

Examples

Experimental program
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Embodiment 1

[0063] A tissue culture propagation method of Tianzhu Gui, which comprises the steps of:

[0064] 1) Selection of female parent material: 50 annual seedlings were selected as the female parent of micropropagation explants;

[0065] The method of cultivating annual seedlings is to collect seeds in the previous year before October 15th, sow them as they are harvested, and use the sand storage method. After the seeds germinate, they are transplanted into containers for cultivation.

[0066] 2) Cultivation environment: Greenhouse cultivation is adopted, the light intensity range is 2000-3000lx, and the temperature range is 12-36°C.

[0067] 3) Moisture control: The leaf surface humidity is controlled at 20%-50%, and the soil humidity is controlled at 40%-90%.

[0068] 4) Cultivation method: use container cultivation, the container is 50cm away from the ground, and 1 / 4 Hoagland's nutrient solution is applied to keep the plant's diameter section tender green, and the axillary buds ...

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Abstract

The invention belongs to the technical field of plant seedling propagation, and discloses a tissue culture propagation method of cinnamomum japonicum. The tissue culture propagation method comprises the following steps of 1) sterilization; 2) preparation of an improved WPM culture medium; 3) induction of adventitious buds; 4) high-voltage electrostatic field treatment; 5) subculture propagation; 6) rooting culture; and 7) transplanting and seedling hardening. According to the tissue culture propagation method, a large number of cinnamomum japonicum root-containing seedlings can be obtained, the survival rate is high, the vacancy of cinnamomum japonicum market resources is effectively solved, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of plant seedling propagation, and in particular relates to a method for tissue culture propagation of osmanthus osmanthus. Background technique [0002] Tianzhugui, also known as Zhejiang camphor ( Cinnamomum chekiangense Nakai) is an evergreen tree of the genus Cinnamomum lauraceae, the adult tree is 15 m high, the bark is taupe, smooth, and the branchlets are glabrous. Leaves leathery, nearly opposite, ovate-oblong or oblong-lanceolate. Osmanthus osmanthus has dense branches and leaves, and the crown is tower-shaped. It is suitable for various planting methods such as cluster planting and solitary planting in landscaping. , bridges, vehicles and furniture, etc.; branches, leaves and bark can extract aromatic oil for making various essences and spices; seeds can be used for oil extraction for making soap and lubricating oil. This tree species is mainly distributed in the southern provinces of my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 芦治国李乃伟殷云龙施钦束晓春
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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