Fat extract without additive components as well as preparation method and application of fat extract

A fat extraction, additive-free technology, applied in the biological field, can solve the problems of unsatisfactory anti-aging effect

Active Publication Date: 2021-02-23
SHANGHAI SEME CELL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although some anti-aging technologies and products have been developed in this field, their anti-aging effects are still unsatisfactory

Method used

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  • Fat extract without additive components as well as preparation method and application of fat extract
  • Fat extract without additive components as well as preparation method and application of fat extract
  • Fat extract without additive components as well as preparation method and application of fat extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1 fat extract preparation

[0110] Fat was obtained from volunteers with informed consent. The fat extraction method is as follows:

[0111] (1) Take the fat obtained by suction or surgical resection, shred it and rinse it with normal saline for 3 times.

[0112] (2) Take the rinsed adipose tissue, put it in a 50ml centrifuge tube (about 30-50ml per tube), put it into a centrifuge and centrifuge at 1200rpm for 3 minutes to obtain a layered mixture.

[0113] (3) For the layered mixture, drain the excess liquid at the bottom and the uppermost layer of fat, and collect the middle layer (ie, the fat layer containing fat cells).

[0114] (4) For the middle layer, use two 10ml injection syringes to connect the three-way pipe to beat about 60-120 times, thereby performing mechanical emulsification, and obtaining a mechanically emulsified fat mixture (also called nanofat).

[0115] (5) Put the mechanically emulsified fat mixture (which can be combined or not) into...

Embodiment 2

[0117] The influence of embodiment 2 fat extracts on human skin fibroblast proliferation

[0118] Human skin fibroblasts were isolated from neonatal foreskin tissue, then inoculated in high-glucose DMEM medium containing 10% fetal bovine serum, and passaged once every 3 days. The 3rd and 4th passage cells were used in the experiment.

[0119] Human skin fibroblasts were inoculated in a 96-well plate at a density of 1,000 cells per well, and fat extracts of different concentrations (1%-5%) were added, and 6 secondary wells were set for each concentration, and used after culturing for 72 hours. The CCK8 kit was used to detect cell proliferation and determine the OD value. The experiment was repeated three times to obtain the average value and standard deviation.

[0120] result:

[0121] Human skin fibroblasts were cultured with different concentrations of fat extracts, CCK-8 detection showed that the number of cells added in the extract group was significantly higher than that...

Embodiment 3

[0123] Example 3 Effect of Fat Extraction on Anti-aging and Oxidative Stress of Skin Tissue

[0124] Treat skin fibroblasts with fat extracts of different concentrations (1%-5%) for 24 hours, ultraviolet rays (100mJ / cm 2 ) after irradiation, the content of reactive oxygen species (ROS) in the cells was detected by flow cytometry.

[0125] After 48 hours after irradiation, the cell cycle was detected by flow cytometry.

[0126] After 72 hours of irradiation, cell proliferation was detected with a CCK8 kit, and senescent cells were stained with beta-gal and phalloidin to detect the degree of aging. In addition, the RNA of the cells was extracted, and the expression of type I collagen was detected by RT-PCR technique.

[0127] result:

[0128] 3.1 ROS content

[0129] Human skin fibroblasts were cultured with fat extracts at different concentrations, intracellular ROS staining was performed immediately after ultraviolet light irradiation, and the accumulation of intracellular...

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Abstract

The invention provides a fat extract without additive components as well as a preparation method and application of the fat extract. Specifically, the invention provides application of the fat extractwithout additive components in preparation of a composition or product having the following one or more applications: (a) promoting proliferation of fibroblasts; (b) promoting the aging resistance ofthe fibroblasts; and (c) promoting the production of type I collagen in the fibroblasts. The invention also provides a preparation method of the fat extract without the additive components, a medicine or cosmetic composition containing the fat extract without the additive components, and a method for culturing fibroblasts in vitro. The fat extract without additive components can efficiently and synergistically inhibit skin aging, prevent fibroblast apoptosis, promote fibroblast proliferation and collagen synthesis, and promote skin rejuvenation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fat extract without additives, its preparation method and application. Background technique [0002] The skin is the body's first protective barrier and plays an important role in resisting external aggression and maintaining a stable internal environment. Skin aging can be divided into endogenous aging and exogenous aging: the programmed aging process controlled by genetic factors is endogenous aging or natural aging, which develops over time and is irresistible; aging caused by environmental factors It is exogenous aging, the most important of which is skin aging caused by repeated exposure to ultraviolet rays, that is, photoaging. [0003] The essence of ultraviolet damage to cells is that the photon energy of ultraviolet rays is absorbed by the atoms or molecules of the cells, causing changes in the electron energy level of the atoms, generating a large number of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/92A61Q19/08A61Q17/04A61K35/35A61P17/00A61P17/06A61P17/02A61P3/10C11B1/00C11B1/02C11B3/00C12N5/071
CPCA61K8/925A61Q19/08A61Q17/04A61K35/35A61P17/00A61P17/06A61P17/02A61P3/10C11B1/00C11B1/02C11B3/001C11B3/008C12N5/0656C12N2500/84C11B1/06C12N2500/36A61K38/19A61K8/92
Inventor 李伟张文杰邓明武徐宇达于子优王祥盛蔡宜佐郑红洁
Owner SHANGHAI SEME CELL TECH CO LTD
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