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SiRNA interfering with cynoglossus semilaevis gametogenesis gene and application of siRNA

A semi-smooth tongue sole and gametogenesis technology, applied in the field of RNA interference, can solve the problems of long sexual maturation cycle, time and labor costs of semi-smooth tongue sole, and achieve the effect of broad application prospects

Active Publication Date: 2021-02-23
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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Problems solved by technology

We knocked down neurl3 in pseudo-male fish by in vivo RNAi experiments and found that the transcription levels of genes related to spermatogenesis were changed, however, in the male fish we were more interested in, we failed to knock down neurl3 Knockdown (Xu, W., Li, H., Dong, Z., et al. Ubiquitin ligase geneurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene.2016, 592: 215–220.)
[0004] Gene knockout is an effective tool for studying gene function, but due to the long sexual maturation cycle of tongue sole (1 year for males and 2 years for females), the time and labor cost to obtain homozygous gene knockout individuals will increase accordingly

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  • SiRNA interfering with cynoglossus semilaevis gametogenesis gene and application of siRNA
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  • SiRNA interfering with cynoglossus semilaevis gametogenesis gene and application of siRNA

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the embodiments. The following descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention to other forms. Changes to equivalent embodiments with equivalent changes. Any simple modifications or equivalent changes made to the following embodiments according to the technical essence of the present invention without departing from the solution content of the present invention fall within the protection scope of the present invention.

[0026] According to the neurl3 sequence and previous results (Xu, W., Li, H., Dong, Z., et al. Ubiquitin ligasegene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis proteinubiquitination. Gene .2016,592:215–220.), we redesigned siRNAs specific to neurl3 (in the present invention, it is defined as si-neurl3), and used siRNA of green fluorescent pr...

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Abstract

The invention relates to the field of RNA interference, in particular to siRNA interfering with a cynoglossus semilaevis gametogenesis gene and application of the siRNA. Specifically, the invention provides optimized siRNA. The siRNA successfully interferes with the gametogenesis gene in ZZ type cynoglossus semilaevis male fish, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of RNA interference, in particular to an siRNA for interfering with the gametogenesis gene of half-smooth tongue sole and an application thereof. Background technique [0002] Cynoglossus semilaevis (Cynoglossus semilaevis) is a kind of flounder, belonging to the Cynoglossus semilaevis family and the genus Cynoglossus, and it is an important marine fish in Northeast Asia. Semi-smooth tongue sole is deeply loved by consumers because of its delicious meat and smooth taste, and has become a rare and precious species in marine cultured fish. There are obvious differences in the growth of female and male in semi-smooth tongue sole, and the growth of female fish can be 2-4 times larger than that of male fish. Therefore, increasing the proportion of female fish in aquaculture is of great significance for increasing production. The half-smooth tongue sole belongs to the ZW sex-determining type, that is, theoretically speakin...

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Application Information

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IPC IPC(8): C12N15/113
CPCC12N15/113C12N2310/14
Inventor 徐文腾陈松林崔忠凯刘洋
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI