Tissue culture and rapid propagation method of new variety 'Shirley' of vertical branch type chionanthus retusus

A technology for tissue culture, rapid propagation and new varieties, which is applied in the field of plant cell engineering to achieve the effects of high multiplication multiple, low mutation rate, and improved reproduction efficiency.

Active Publication Date: 2021-03-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

'Xueli' is a newly cultivated weeping tassel tree variety, and there is no research or report on its regeneration by tissue culture

Method used

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  • Tissue culture and rapid propagation method of new variety 'Shirley' of vertical branch type chionanthus retusus
  • Tissue culture and rapid propagation method of new variety 'Shirley' of vertical branch type chionanthus retusus
  • Tissue culture and rapid propagation method of new variety 'Shirley' of vertical branch type chionanthus retusus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Based on WPM, add 30g / L sucrose and 6g / L agar, and add different concentration gradients of KT (0.1, 0.5, 1.0mg / L), 6-BA (1.0, 1.5, 2.0mg / L) Two-factor random block design, experimental design shown in Table 1. 30 flasks were inoculated with each medium, with 2 explants per flask. The growth height of stem segments, the condition of new leaves and the proliferation of adventitious buds were observed every week (proliferation coefficient=number of new shoots multiplied / inoculated quantity). Culture conditions: The culture temperature is 24°C±2°C, the light intensity is 2500-3000lx, the light time is 12h / d, and the pH of the medium is 5.8-6.2.

[0043] Table 1: Effects of different growth regulators on stem segment differentiation of 'Xueli' (observation results at 35 days)

[0044]

[0045] After culturing for 35 days, it was observed that the combination of growth regulators in group 5 had the best effect on promoting the differentiation of 'Xueli' stem segments, t...

Embodiment 2

[0047] Based on WPM, add 30g / L sucrose, 6g / L agar, 1.5mg / L 6-BA, 0.5mg / L KT, by adding different concentrations of GA 3 (0.5-4mg / L) to carry out single factor experiment, experimental design is shown in Table 2. Each medium was used to inoculate 30 bottles, and each bottle had 2 stem segments cultured with the medium that induced stem segment differentiation. Observe and record the elongation height of stem segment and seedling height every week. Culture conditions: culture temperature is 24°C±2°C, light intensity is 2500-3000lx, light time is 12h / d, medium pH is 5.8-6.2.

[0048] After culturing for 15 days, it was observed that group 3 (i.e. adding 2.0mg / L of GA 3 ) had the best effect on promoting the elongation of the stem section of 'Xueli', and the stem section of tassel tree could grow by 3.01cm (Table 2 and image 3 ).

[0049] Table 2: GA of stem segments of ‘Xueli’ 3 single factor elongation test

[0050]

Embodiment 3

[0052] With WPM as the basic medium, add 30g / L sucrose and 6g / L agar, add different concentration gradients of IBA (0.1-1.5mg / L), NAA (0.1-1.5mg / L) to carry out two-factor random block design , see Table 3 for the experimental design. Observe and record rooting performance every week. Culture conditions: culture temperature is 24°C±2°C, light intensity is 2500-3000lx, light time is 12h / d, medium pH 5.8-6.2.

[0053] Table 3: Effects of different plant growth regulators on rooting of 'Xueli'

[0054]

[0055] It was observed that the combination of growth regulators in group 8 had the best effect on promoting rooting, and the root primordium was formed after about 15 days of culture. After 35 days of culture, the root length reached 5.1 cm, and the rooting rate reached 62.79%.

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Abstract

The invention discloses a tissue culture rapid propagation method of a new variety 'Shirley' of a vertical branch type chionanthus retusus. The method comprises the following steps of (1) cutting a tender stem with the length of 2-2.5 cm from a 'Shirley' stock plant as a test material, inoculating the test material into a culture medium for inducing stem differentiation, and inducing adventitiousbud differentiation and proliferation; (2) when the test material in the step (1) has 4-5 new leaves and is differentiated to form 1-2 adventitious buds, transferring the test material into the culture medium for inducing stem elongation so as to promote stem elongation; (3) when the stem of the test material in the step (2) elongates to 3-5cm, transferring the stem into a rooting culture medium,and carrying out adventitious root induction; and (4) hardening and transplanting rooted seedlings. When the method disclosed by the invention is used for propagating the 'Shirley', the new variety 'Shirley' can be popularized in a short time, so that the new variety 'Shirley' can be rapidly applied to greening practice. A theoretical basis is provided for establishing a rapid propagation system with stable and efficient chionanthus retusus characters and a subsequent genetic transformation experiment.

Description

technical field [0001] The invention relates to the technical field of plant cell engineering, in particular to a tissue culture and rapid propagation method for a new weeping tassel tree variety 'Xueli'. Background technique [0002] Tassel tree (Chionanthus retusus Lindl.et Paxt.) belongs to Oleaceae (Oleaceae) Chionanthus plant, deciduous trees or shrubs, the height of adult trees can reach 20m. The bark of young trees is mostly light yellow, the branchlets are cylindrical, taupe, without hair, the young branches are light yellow or brown, sparsely or densely covered with pubescence. Adult tree bark gray-brown, longitudinally fissured, with obvious grooves and ridges. The leaves are simple, opposite, nearly leathery, elliptic or oblong, with slightly rolled margins, entire margins or small serrations, blunt or sharp leaf tips, cuneate or heart-shaped leaf bases. Panicles are born at the top of side branches, unisexual dioecious or bisexual flowers, corolla white, 4-part...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李际红邢世岩刘会香周文玲王如月郭海丽王锦楠刘佳庚侯丽丽
Owner SHANDONG AGRICULTURAL UNIVERSITY
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