Pharmaceutical composition of guvacine hydrochloride and medical application of pharmaceutical composition
A kind of technology of nor arecoline and composition, applied in the field of chemical medicine, can solve the problems such as anti-breast cancer that no nor arecoline and alfalfa acid have been seen
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Embodiment 1
[0013] 1. Experimental materials
[0014] Human breast cancer MCF-7 cells were purchased from ATCC and cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C, 5% CO 2 Routine culture in a cell culture incubator.
[0015] RPMI-1640 medium and fetal bovine serum were purchased from Gibco, USA.
[0016] Norcaline and alfalfaic acid were purchased from Chengdu Desite Biotechnology Co., Ltd., with a purity of ≥98%.
[0017] 2. Experimental methods and results
[0018] Human breast cancer MCF-7 cells in the logarithmic growth phase were digested and resuspended to make a cell suspension, and seeded in a 96-well plate, 1×10 per well 4 1 cell, 200 μL, 37°C, 5% CO 2 After 24 hours of conditioned culture, replace it with RPMI-1640 medium containing different concentrations of different drugs and 10% fetal bovine serum according to Table 1 and continue to culture for 48 hours. At the same time, a blank control group without drugs was set, with 5 duplicate wells for each. ...
Embodiment 2
[0027] A pharmaceutical composition of norarecoline, which is composed of norarecoline and alfalfaic acid in a mass ratio of 2:1. Based on the composition, an anti-breast cancer pharmaceutical preparation is prepared, and the active ingredients are norarecoline and alfalfaic acid in a mass ratio of 2:1.
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