Detection method of bupleurum chinense and south bupleurum formula granules
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A technology of formula particles and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as difficulty, long detection time, unintuitiveness, etc., and achieve the effect of improving accuracy, simple method and good separation degree.
Active Publication Date: 2021-06-01
BEIJING KANGRENTANG PHARMA
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[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects of the prior art methods for detecting the formula granules of North Bupleurum and South Bupleurum that are difficult, not intuitive, and take a long time to provide a formula granule of North Bupleurum and South Bupleurum detection method
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Embodiment 1
[0059] This embodiment provides a method for detecting the formula granules of North Bupleurum and South Bupleurum, comprising the following steps,
[0060] Preparation of the reference substance solution: take saikosaponin a, weigh it accurately, add the first solvent methanol to make a 0.75 μg / ml reference substance solution;
[0061] Preparation of reference standard medicinal material solution: take 0.5g of northern Bupleurum and southern Bupleurum as reference medicinal materials, accurately weigh them, put them in a conical flask, add 25ml of water precisely, seal them tightly, weigh them, heat and reflux for 30min, and filter to obtain the first One filtrate, take 20ml of the first filtrate, recycle the solvent water until dry to obtain the first residue, precisely add 25ml of 5% concentrated ammonia and 50% ethanol, seal it tightly, weigh it, ultrasonic (power 250W, frequency 40kHz) for 30min, take it out, put Cool, weigh again, make up the lost weight with 50% ethanol...
Embodiment 2
[0102] This embodiment is an investigation into the methodology of ultra-high performance liquid chromatography
[0103] 1 Repeated investigation
[0104] Prepare 6 parts of need testing solution in parallel according to the preparation method of need testing solution in Example 1, test according to the above-mentioned method chromatographic conditions and assay method, obtain characteristic collection of spectra, take peak 3 saikosaponin a as reference peak, calculate relative peak Area and relative retention time, the relative retention time RSD of each characteristic peak is in the range of 0-0.1%, and the RSD of the relative peak area is 0.6%-2.2%, indicating that the repeatability of the characteristic spectrum is good.
[0105] 2 Precision inspection
[0106] Prepare 6 parts of need testing solutions in parallel according to the preparation method of need testing solution in Example 1, adopt different instruments to test according to the above-mentioned method chromatog...
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Abstract
The invention belongs to the technical field of medicine detection, and particularly relates to a detection method of bupleurum chinense and south bupleurum formula granules. The detection method comprises the following steps: firstly comparing the chromatogram of a test sample solution with the chromatogram of a reference standard medicinal material solution, then further detecting according to the ratio beta of the peak area of saikoside c to the peak area of saikoside a, firstly carrying out preliminary detection on a test sample, and then further detecting. The bupleurum chinense and south bupleurum formula granules are detected by a method for determining the peak area ratio of saikosaponin a to saikosaponin c, and the UPLC method is relatively good in detection separation degree, greatly shortens the detection time, is simple, convenient and intuitive, and has good repeatability and accuracy. According to the method, the standard control medicinal material solution is used as an evaluation standard and is consistent with a standard control substance in clinical application, whether the formula granules are bupleurum chinense formula granules or not can be accurately detected by controlling all links of the detection method, and the method has practical application value.
Description
technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for detecting formula granules of northern bupleurum and southern bupleurum. Background technique [0002] Bupleurum is the dried root of Bupleurum chinense DC. or Bupleurum scorzonerifolium Willd. of Umbelliferae, and is commonly called "North Bupleurum" and "South Bupleurum" according to their characters. First recorded in "Shen Nong's Materia Medica", it tastes pungent, bitter, and slightly cold, and belongs to the liver, gallbladder, and lung meridians. Nan Bupleurum is good at raising yang and dispelling evils, soothes the liver and relieves stagnation, and has a light smell. It is most suitable for people with exogenous pathogenesis on the surface and depression of Qingyang. Bupleurum bupleurum is good at dispelling heat and expelling pathogenic factors, promoting the old and bringing forth the new. It has a good dredging and resolving effect on...
Claims
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