A method for establishing the hplc fingerprint of medicinal material Sanbai herb
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A technique for fingerprinting and establishing methods, which is applied in the direction of material separation, analysis of materials, instruments, etc., can solve the problems of low detection accuracy and sensitivity, long detection time, large measurement errors, etc., to shorten detection time and repeat Good performance and stability, reliable experimental results
Active Publication Date: 2022-03-22
GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM) +1
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[0003] At present, the research on the quality control of Sanbai grass is not perfect, and most of them focus on the quality evaluation of single chemical components such as tripaiquidone and licarin A. There are also related reports on the study of fingerprints, but they are not perfect
Nowadays, fingerprints are often used to detect the components of Chinese herbal medicines, but there are large measurement errors, such as differences in various factors such as detection instruments, mobile phases, and environmental conditions, and the time required for detection is too long, and the amount of sample required is large. Accuracy and sensitivity are not high
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[0038] 1.2 Reference substances: hyperin (111521-201205), rutin (100080-201409), quercitrin (111538-200504) were purchased by China Food and Drug Administration; Brutuyone, (-)-Braquityl Methyl Ether, Tribakuydone, Tribakuyol I, Licarin A, 4-O-Desmethyl Manasatin B, Manasatin B , Licarin B was provided by the team of Professor Xu Jun of Sun Yat-sen University; methanol was chromatographically pure (Thermo Fisher Scientific (China) Co., Ltd.); water was Watsons distilled water; the rest of the reagents were analytically pure.
[0039]...
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Abstract
The invention discloses a method for establishing the HPLC fingerprint of the medicinal material Sanbaicao, which comprises the following steps: Step 1, preparation of the test solution: take the sample powder of Sanbaicao, add 80% methanol, and extract by reflux, Use 80% methanol to make up the lost quality, filter, and get the filtrate to obtain the test solution; step 2, the establishment of fingerprints: use high performance liquid chromatography to detect the test solution, and establish the test solution The fingerprint of the solution. The invention sensitively performs qualitative and quantitative analysis on samples, better reflects the similarity of common peaks, better detection separation, reliable experimental results, good repeatability and stability, high precision, and greatly shortens the detection time. time.
Description
technical field [0001] The invention relates to the field of fingerprints, in particular to a method for establishing an HPLC fingerprint of a medicinal material of Sanbai herb. Background technique [0002] Sanbaicao comes from Saururus chinensis (Lour.) Baill., the dried aerial part of the family Tribaceae, and is used as medicine. It is sweet, pungent, cold in nature, and returns to the lung and bladder meridian; it has the effect of diuresis, swelling, heat-clearing and detoxification ; For the treatment of edema, difficulty urinating, leaching pain, leukorrhea; external treatment of sores, swollen toxins, eczema, etc. Modern studies have reported that Sanbai herb contains volatile oils, flavonoids, lignans, alkaloids, tannins and other components, and has been found to have anti-inflammatory, detoxification, hypoglycemic, liver-protecting, diuretic and other activities. The lignans in Sanbaicao have various pharmacological activities, such as anti-inflammation and anti...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
Inventor 毕晓黎徐峻顾琼江洁怡蒋富强胥爱丽陈伟韬李养学
Owner GUANGDONG SECOND TRADITIONAL CHINESE MEDICINE HOSPITAL (GUANGDONG PROVINCE ENG TECH RES INST OF TCM)
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