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Method for detecting bupleurum chinense and vinegar-processed bupleurum chinense formula granules

A technology of formula granules and vinegar of Bupleurum chinensis is applied in measuring devices, instruments, scientific instruments and other directions, which can solve the problems of difficult detection methods and low accuracy, and achieve shortened detection time, good repeatability and accuracy, and ease of use. effect of operation

Inactive Publication Date: 2021-06-01
BEIJING KANGRENTANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of difficulty and low accuracy in the detection method of Vinegar Bei Bupleurum in the prior art, thereby providing a method for detecting Bei Bupleurum and Vinegar Bei Bupleurum formula granules

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  • Method for detecting bupleurum chinense and vinegar-processed bupleurum chinense formula granules
  • Method for detecting bupleurum chinense and vinegar-processed bupleurum chinense formula granules
  • Method for detecting bupleurum chinense and vinegar-processed bupleurum chinense formula granules

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Embodiment 1

[0060] This embodiment provides a method for detecting Bei Bupleurum and Vinegar Bei Bupleurum formula granules, comprising the following steps,

[0061] Preparation of the reference substance solution: take saikosaponins a, b1, b2, c and f respectively, weigh them accurately, add the first solvent methanol to make a 0.75 μg / ml reference substance solution;

[0062] Preparation of the reference standard medicinal material solution: take 0.5g of Bupleurum bupleurum reference standard medicinal material, accurately weigh it, put it in a conical flask, add 25ml of water precisely, seal it tightly, weigh it, heat and reflux for 30min, filter to obtain the first filtrate, take Continue filtrate 20ml, recover the solvent water to obtain the first residue, then accurately add 25ml of 50% ethanol containing 5% concentrated ammonia to the first residue, plug it tightly, weigh it, ultrasonic (power 250W, frequency 40kHz) for 30min, take out , cooled, weighed, supplemented the lost weigh...

Embodiment 2

[0099] This embodiment provides the methodological investigation of the detection method of embodiment 1

[0100] 1. Repeatability

[0101] According to the preparation method of need testing solution, prepare 6 parts of need testing solution in parallel, test according to the above-mentioned chromatographic conditions and assay method, obtain characteristic collection, take No. 3 peak as reference peak, calculate the relative concentration in different need testing solutions Peak area and relative retention time, the results show that the relative retention time RSD of each characteristic peak is 0.0% to 0.1%, and the RSD of the relative peak area is 0.6% to 2.2%, indicating that the UHPLC has good repeatability of the spectrum .

[0102] 2. Precision

[0103] According to the preparation method of need testing solution, prepare 6 parts of need testing solution in parallel, adopt different instrument to test according to above-mentioned chromatographic conditions and assay ...

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Abstract

The invention belongs to the technical field of medicine detection, and particularly relates to a method for detecting bupleurum chinense and vinegar-processed bupleurum chinense formula granules. The ultra-high performance liquid chromatography (UPLC) method is good in detection and separation degree, firstly, the characteristic peaks are compared, then the bupleurum chinense and vinegar-processed bupleurum chinense formula granules are detected by taking the ratio of saikoside b1 to saikoside a and saikoside b1 as critical points, the method is visual, accurate and easy to operate, the detection time is greatly shortened, and good repeatability and accuracy are achieved; according to the method, the conversion condition of saikoside b1 and saikoside a is used as a critical point because after vinegar-processed bupleurum chinense is processed, primary saponin is converted into secondary saponin, the conversion degree is higher than that of bupleurum chinense, and meanwhile, under the same standard condition, the difference of the conversion degree is kept, the relative content of the converted secondary saponin b1 in the vinegar-processed bupleurum chinense formula granules and bupleurum chinense formula granules is high, and the relative ratio of the secondary saponin b1 is used as a detection point, so that the method is accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for detecting formula granules of Bei Bupleurum and Vinegar Bei Bupleurum. Background technique [0002] Bupleurum is the dried root of Bupleurum chinense DC. or Bupleurum scorzonerifolium Willd. of Umbelliferae. According to different characters, they are commonly called "North Bupleurum" and "South Bupleurum". First recorded in "Shen Nong's Materia Medica", it tastes pungent, bitter, and slightly cold, and returns to the liver, gallbladder, and lung meridians. It has the functions of evacuating and reducing fever, soothing the liver and relieving stagnation, and promoting Yang Qi. It is mainly used for cold and fever, alternating cold and heat, distending pain in the chest and hypochondrium, irregular menstruation, prolapse of the uterus, and prolapse of the anus. Bupleurum is a commonly used traditional Chinese medicinal material and has a long his...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N30/88G01N2030/027G01N2030/062G01N2030/8809
Inventor 付静张志强沈建梅杜微波吉艳慧陈敬然
Owner BEIJING KANGRENTANG PHARMA
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