Method for obtaining epiphytic bacteria-free ulva lactuca

A technology of Ulva and epiphytes, applied in the field of algae research, can solve the problem of not being able to obtain Ulva without epiphytes, and achieve the effect of enriching knowledge

Active Publication Date: 2021-06-11
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for obtaining Ulva without epiphytic bacteria, which solves the problem that the prior art cannot obtain Ulva without epiphytic bacteria

Method used

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  • Method for obtaining epiphytic bacteria-free ulva lactuca
  • Method for obtaining epiphytic bacteria-free ulva lactuca
  • Method for obtaining epiphytic bacteria-free ulva lactuca

Examples

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Embodiment 1

[0053] A method of obtaining Ulva without epiphytic bacteria, comprising:

[0054] A. Weigh 4g of fresh Ulva, place the weighed algae in sterilized in-situ seawater, and scrub the surface of the algae with a sterilized brush;

[0055] B. Put the Ulva after preliminary treatment into the Erlenmeyer flask, add 600ml sterile seawater and 20ml multi-enzyme cleaning agent, the multi-enzyme cleaning agent includes protease, lipase, amylase, surfactant, and this embodiment uses commercially available 3M fast and high-efficiency multi-enzyme cleaning solution (Minnesota Mining Manufacturing Medical Equipment Co., Ltd.), placed on a shaker at 180rpm for 2h, ultrasonically cleaned for 10mim-15min;

[0056] C. Take half of the Ulva treated with multi-enzyme cleaning agent, wash it with sterile sea water for 3 times, place it in an ultrasonic breaker (Ultrasonic Homogenizer JY92-ⅡN) at 300w, 10s: 10s ultrasonic break for 20mins, pour the broken liquid into Centrifuge the tube at 4°C, 600...

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Abstract

The invention belongs to the field of algae research, and discloses a method for obtaining epiphytic bacteria-free ulva lactuca. The method comprises the following steps that A, fresh ulva lactuca is placed in sterilized in-situ seawater, and the surface of the fresh ulva lactuca is brushed by using a sterilized writing brush to obtain a brushed ulva lactuca frond; B, the brushed ulva lactuca is mixed with a multi-enzyme cleaning agent, and ultrasonic cleaning is carried out to obtain enzyme-washed ulva lactuca fronds; C, the enzyme-washed ulva lactuca is mixed with antibiotics, and sterilizing is carried out to obtain sterilized ulva lactuca fronds; and D, the sterilized ulva frond is put into a sterilized culture medium to be cultured, and the non-epiphytic ulva is obtained. The problem that in the prior art, the normally-growing non-epiphytic ulva cannot be obtained is solved.

Description

technical field [0001] The invention belongs to the field of algae research, in particular to a method for obtaining Ulva without epiphytic bacteria. Background technique [0002] Algae has achieved unprecedented development from theory to technology, but compared with other disciplines, it started late and its development is relatively lagging behind. The inability to obtain some algae-free strains is the main factor restricting the research of algae. The study of algae is largely limited by the difficulty of obtaining bacteria-free strains of the target algal species. [0003] As far as Ulva is concerned, attached bacteria play an important role in the morphogenesis of Ulva. Because there is often a special relationship between microorganisms or symbiosis, endophytic, parasitic, etc., once this relationship is destroyed, it will be unfavorable for the growth of algae and bring difficulties to the preparation of sterile strains; sometimes it is Because conventional method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00C11D3/386
CPCA01G33/00C11D3/38618C11D3/38627Y02A40/80
Inventor 陈思源陈善文
Owner SHANTOU UNIV
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