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Target component knockout-based traditional Chinese medicine efficacy component identification method and application

A technology for target ingredients and traditional Chinese medicine, which is applied in the field of pharmaceutical technology and drug screening, and can solve the problems of high throughput and high precision, which are difficult to take into account the overall view and system view of traditional Chinese medicine.

Active Publication Date: 2021-06-22
FUJIAN HEALTH COLLEGE
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the existing separation and analysis technology is difficult to take into account the holistic view and the systematic view of traditional Chinese medicine in terms of high throughput and high precision, the inventors provide a method for the identification of pharmacodynamic groups of traditional Chinese medicine based on the knockout of target components. The method, technical scheme is as follows:

Method used

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  • Target component knockout-based traditional Chinese medicine efficacy component identification method and application
  • Target component knockout-based traditional Chinese medicine efficacy component identification method and application
  • Target component knockout-based traditional Chinese medicine efficacy component identification method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of Indigo Naturalis Extract Sample Powder

[0041] Precisely weigh 25g of each of six parts of Qingdai powder, put them in a beaker, measure 300ml of N,N-dimethylformamide (DMF) and soak for 30min, press ultrasonic power 700W, ultrasonic temperature 50℃, ultrasonic time 60min, ultrasonic extraction frequency is Three times, collect the Qingdai extract, filter, transfer it to a rotary steamer, and rotate at a speed of 140n·min -1 , the water bath heating temperature is 65°C, and the cooling temperature is -4°C for concentration, and the Indigo Naturalis extract concentrate is placed in a vacuum oven to prepare a powder.

[0042] The experimentally obtained sample powder is about 2.2 g, and the calculated yield is about 1.47%.

[0043] 2. Preparation of Indigo Naturalis Extract Knockout Fraction

[0044] 1. Semi-preparative liquid chromatography qualitative analysis

[0045] Accurately weigh 10.00 mg each of tryptanthrin, indirubin and isorhamnetin refer...

Embodiment 2

[0083] Preparation of Indigo Naturalis Extract Powder: ultrasonically extract the chemical components in Indigo Naturalis, the ultrasonic conditions are power 700W, temperature 50°C, time 60min, times 3 times; the extraction solution is concentrated, the condition is speed 140n·min -1 , the water bath heating temperature is 65°C, and the cooling temperature is -4°C; the Qingdai extract is prepared into powder and set aside;

[0084] Preparation of Knockout Fractions of Indigo Naturalis Extract: The target components indirubin, tryptanthrin and isorhamnetin were separated by semi-preparative liquid chromatography. Chromatographic conditions: using Shim-pack GISS C18 chromatographic column, the flow rate is 3mL min -1 , the detection wavelength is 290nm, and the sample volume is 4mL; elution conditions: gradient elution with 0.1% glacial acetic acid-methanol as the mobile phase. The elution program is shown in Table 1. Such as image 3 Fractions free of indirubin, tryptanthri...

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Abstract

The invention discloses a target component knockout-based traditional Chinese medicine effective component identification method and application, and belongs to the field of pharmaceutical technology and medicine screening. The method comprises the following steps: extracting chemical components of the traditional Chinese medicine, knocking out target components to prepare different component groups, identifying the knock-out components, evaluating the knock-out effect, quantifying the knock-out components, truly reducing the proportion of the different component groups in the traditional Chinese medicine, and determining a main pharmacodynamic component group. The indigo naturalis extract knockout component with the anti-leukemia effect is prepared and screened out. Basic support is provided for follow-up indigo naturalis quality control, anti-leukemia drug effect research and pharmaceutical preparations, and the traditional Chinese medicine composition has reference and popularization value for other drugs.

Description

technical field [0001] The invention belongs to the field of pharmaceutical technology and drug screening, and in particular relates to a pharmacodynamic group identification or screening method of traditional Chinese medicine based on the combination of target knockout components and quantitative methods, and its application in Qingdai anti-leukemia. Background technique [0002] Leukemia is a malignant disease of the hematopoietic system and one of the top ten malignant tumors that cause death in Chinese residents. Under the guidance of traditional Chinese medicine theory, the traditional Chinese medicine compound "Danggui Longhui Pill" and "Huangdai Tablet" can effectively treat leukemia. Among the above compound recipes, Qingdai is the most important anti-leukemia Chinese herbal medicine. It is the leaves or stems of the Acanthaceae plant Aphicacanthuscusia (Nees) Bremek, the Polygonum tinctonum Ait. or the Cruciferous plant Isatisindigotica Fort. The processed dry powd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/146
Inventor 黄慧李泳宁戴雅彬张月芬
Owner FUJIAN HEALTH COLLEGE
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