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Application of black phosphorus nanosheet in preparation of medicine for treating Parkinson's disease

A Parkinson's disease, nanosheet technology, applied in drug delivery, drug combination, phosphorus compound active ingredients, etc., can solve the problem of limited efficiency of brain-targeted drug delivery, difficulty in reaching PD lesion areas, difficulty in brain-targeted drug delivery, etc. problem, achieve the effect of improving brain targeting efficiency and increasing permeability

Inactive Publication Date: 2021-07-23
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the antioxidants with the ability to scavenge excess ROS do not have the ability to cross the blood-brain barrier, and it is difficult to reach the PD lesion area
Although with the development of nanotechnology, drugs can be delivered across the blood-brain barrier through various methods such as transporter-mediated and receptor-mediated, but the brain-targeted drug delivery efficiency of these methods is limited, and it is difficult to achieve efficient drug delivery. brain-targeted drug delivery

Method used

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  • Application of black phosphorus nanosheet in preparation of medicine for treating Parkinson's disease
  • Application of black phosphorus nanosheet in preparation of medicine for treating Parkinson's disease
  • Application of black phosphorus nanosheet in preparation of medicine for treating Parkinson's disease

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Embodiment 1

[0035] An embodiment of the black phosphorus nanosheets of the present invention, the present embodiment provides a preparation method of polyethylene glycol-modified black phosphorus nanosheets, the preparation method is: 10 mg of polyethylene glycol (molecular weight = 2000) was dispersed in 10 mg black phosphorus nanosheet solution, the black phosphorus nanosheet solution was 200 μg / mL black phosphorus nanosheet aqueous solution, stirred at 850 rpm overnight (8-14 h) in the dark, then centrifuged at 10000 rpm for 30 minutes , remove unloaded polyethylene glycol, resuspend the final product in PBS (1×, pH=7.4), and prepare black phosphorus nanosheets modified with 1 mg / mL polyethylene glycol.

[0036] Depend on figure 1 Visible, the polyethylene glycol modified black phosphorus nanosheet that the present invention makes, its infrared spectrum is in 2900-3000cm -1 The absorption peak at the place is the characteristic absorption peak of methylene, and the scanning transmissi...

Embodiment 2

[0039] An embodiment of the black phosphorus nanosheets of the present invention, this embodiment provides the photothermal behavior of the black phosphorus nanosheets in Example 1. In order to measure the photothermal stability of black phosphorus nanosheet in embodiment 1, it is placed under near-infrared light irradiation (808nm, 0.5W / cm 2 , 8min) to make it produce photothermal effect, then stop the near-infrared irradiation, cool for 8min, and then irradiate with the same near-infrared light again, and this process is repeated five times. Such as image 3 As shown in A, after multiple light-cooling treatments, the black phosphorus nanosheets still have a high photothermal conversion ability.

[0040] Centrifuge the black phosphorus nanosheet solution at 18,000rmp for 30min, remove the supernatant, and then dry it in a vacuum environment at 80°C for 2h, weigh different weights of blackphosphorus nanosheets and resuspend them in PBS to make a gradient Concentration (1, 2,...

Embodiment 3

[0046] An embodiment of the black phosphorus nanosheets of the present invention, this embodiment provides the in vitro neuroprotective effect of the black phosphorus nanosheets described in Example 1. The neuroblastoma cell SH-Sy5y cells (ATCC) were divided into 5×10 3 The cells / mL concentration were inoculated in 96-well plates, incubated for 24 hours, then added black phosphorus nanosheets for pretreatment for 2 hours, and then added 2mM MPP+ to induce neurotoxicity. After 36 hours, the cell viability was evaluated by CCK-8, and live / dead cells were stained with AM and PI dyes, and the final results were as follows: Figure 4 As shown, black phosphorus nanosheets can significantly reduce the neurotoxicity induced by MPP+, increase the survival rate of SH-Sy5y cells, and the surface black phosphorus nanosheets have anti-Parkinson's disease effects in vitro.

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Abstract

The invention relates to application of a black phosphorus nanosheet in preparation of a medicine for treating the Parkinson's disease. The black phosphorus nanosheet is a black phosphorus nanosheet of which the surface is modified with polyethylene glycol. The black phosphorus nanosheet has photothermal conversion performance, can improve blood-brain barrier permeability through a photothermal effect, and can improve the brain-targeted delivery efficiency of the black phosphorus nanosheet. In addition, the black phosphorus nanosheet also has oxidation resistance, can remove excessive active oxygen in the Parkinson's disease pathological state, has a nano biological effect of resisting the Parkinson's disease, and has a wide application prospect in future clinical practice.

Description

technical field [0001] The invention belongs to the application field of medical materials, and in particular relates to the application of black phosphorus nanosheets in the preparation of drugs for treating Parkinson's disease. Background technique [0002] Parkinson's disease (PD) is the second most common neurodegenerative disease. The clinical manifestations are mainly divided into motor disorders such as tremor and gait instability, and non-motor disorders such as insomnia and anxiety. The main pathological feature is the presence of Lewy bodies. Production and loss of dopaminergic neurons. At present, the number of PD patients in my country has exceeded 1.4 million, and with the aging of the population, the number of PD patients will further increase. However, the existing drugs can only alleviate its motor impairment, and there is still a lack of effective treatment strategies for this disease in clinic. [0003] In the pathological mechanism of PD, reactive oxygen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/42A61K47/69A61K47/60A61K41/00A61K9/08A61P25/16
CPCA61K33/42A61K47/6953A61K47/60A61K41/0052A61K9/0019A61K9/08A61P25/16
Inventor 陈桐楷张晗程国旺罗景山李中俊刘瑶
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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