Pharmaceutical composition for liver injury protection and application thereof
A liver injury and composition technology, applied in the field of pharmaceutical compositions for liver injury protection, to achieve the effects of reducing hepatic steatosis, reducing the number of fibroblasts and lymphocytes, and good anti-hepatic fibrosis activity
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Embodiment 1
[0036] The preparation of embodiment 1 Zhengyuanfang water extract
[0037]The traditional Chinese medicine composition preparation is prepared according to the following raw materials in parts by weight: 20 parts of American ginseng, 30 parts of Agrimony, 10 parts of Angelica sinensis, 15 parts of Chonglou, 15 parts of Nansha ginseng, and 10 parts of Platycodon grandiflora, decocting 3 times, each Add 10 times the amount of water for the first time, decoct for 1.5 hours each time for the first two times, and decoct for 1 hour for the third time, combine the water decoctions of the three decoctions, and concentrate under vacuum at 60°C to a relative density of 1.05-1.15 Thick paste, spray-dried, collect the fine powder and all the medicinal powder adhered to the inner wall of the spray dryer, mix well, and obtain the water extract of Zhengyuanfang.
[0038] The American ginseng adopted in the present invention is cool in nature, sweet in taste, slightly bitter, and returns to ...
Embodiment 2
[0039] Example 2 Effect of Zhengyuan Fang water extract on the viability of L02 human normal liver cells
[0040] L02 cells were divided into 1.5×10 5 Each / mL density is inoculated in 96 well plate, overnight, add Zhengyuanfang aqueous extract, make the working concentration of the Zhengyuanfang aqueous extract in each hole be 20, 50, 100, 200, 500, 1000 μ g / mL, After 24 hours, MTT was added to measure the cell viability, and the effect of different concentrations of Zhengyuanfang water extract on the viability of L02 normal human liver cells was determined. For the bar graph of the survival rate of L02 cells, see figure 1 , the present invention found that the water extract of Zhengyuan Fang had a proliferation-promoting effect on human normal liver cell L02 in the range of 100 μg / mL to 500 μg / mL, and the pro-proliferation effect was significant at 500 μg / mL.
Embodiment 3
[0041] Embodiment 3 Zhengyuanfang water extract is to H 2 o 2 Protective effect of induced oxidative damage in L02 hepatocytes
[0042] By the action concentration range of the suitable Zhengyuanfang water extract of result screening in embodiment 2, further pass H 2 o 2 Induce and construct the liver cell oxidative damage model, and measure the survival rate of cells in each group by MTT. For the results, see figure 2 , the present invention finds that Zhengyuan Fang has obvious pro-proliferation effect on normal human liver cells, and hydrogen peroxide H 2 o 2 Induce L02 liver cells to cause oxidative damage model, set H 2 o 2 The concentration was 250μM to injure, after administration, it was found that Zhengyuan Fang had a protective effect on the liver cells damaged by hydrogen peroxide. The living cells in each group were stained by Hoechst 33342 / PI double staining, and the stained cells were observed under the Hoechst33342 fluorescence channel with a fluorescenc...
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