SNP molecular marker related to pig residual feed intake on pig chromosome 1 and application thereof

A technology of molecular markers and chromosomes, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., to improve the economic profit of enterprises, speed up the breeding process, and reduce food intake.

Active Publication Date: 2021-11-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, residual feed intake is an important trait in the genetic improvement work of breeding pigs, but few molecular markers related to residual feed intake have been identified in Duroc pig populations

Method used

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  • SNP molecular marker related to pig residual feed intake on pig chromosome 1 and application thereof
  • SNP molecular marker related to pig residual feed intake on pig chromosome 1 and application thereof
  • SNP molecular marker related to pig residual feed intake on pig chromosome 1 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] (1) Experimental animals

[0032] The group of experimental pigs used in the present invention is the purebred plus line Duroc head of the breeding pig branch of Wen's Food Group Co., Ltd., which is the core group of the breeding pig branch.

[0033] In this experiment, a total of 1265 Duroc pigs were selected in the resource group, and the data were collected with the production performance measurement system of Austrian pigs when each individual was between 30kg and 100kg in weight.

[0034] The pigs in this experiment had free access to food and water, and the entire feeding method and feeding conditions were always consistent, which was a conventional method.

[0035] Duroc pigs have a small number of litters, with an average of only 9 to 10 pigs in a large group, but they grow fast, have a high feed conversion rate, a high carcass lean meat rate, a high intramuscular fat content, and strong stress resistance. Domestic commercial pig production has established a re...

Embodiment 2

[0058] Example 2 Target DNA sequence amplification and sequencing

[0059] (1) Primer design

[0060] The DNA sequence of SEQ ID NO: 1 on pig chromosome 14 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers, entrust Sangon Bioengineering (Shanghai) Co., Ltd. to synthesize primers. The DNA sequences of the designed primers are as follows:

[0061] P001-F: 5'-GGGATGGAGCAGAGAGGTAGGAAC-3',

[0062] P002-R: 5'-TCTATACCCAGGCCGAGAGCAAAG-3';

[0063] (2) PCR amplification

[0064] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0065] (3) DNA sequence d...

Embodiment 3

[0069] Example 3 SNP site g.123C>A effect analysis of molecular markers

[0070] According to Table 1 and figure 2 It can be seen that for the remaining feed intake, the effect of the SNP site g.123C>A dominant allele (TT) significantly reduces the daily feed intake by 141g / day on average compared with the CC phenotype. Calculated on the basis of 165 days when the body weight reaches 100kg, 23.27kg of feed can be saved per pig. For example, it is estimated that 2,327 tons of feed can be saved in a pig farm with an annual slaughter of 100,000 heads. According to the feed cost of 3.5 yuan per kilogram, the feed cost can be saved by 810 million yuan. Therefore, through molecular marker-assisted selection and gradually eliminating the pigs with genotype CC in the population, the allele frequency of allele A can be significantly increased, the remaining feed intake of the population can be reduced, and the feed utilization efficiency of pigs can be improved. Improvement, which w...

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to pig residual feed intake on a pig chromosome 1 and application thereof, the locus of the SNP molecular marker is the 34718602 nucleotide locus on the chromosome 1 of the international pig reference genome version 11.1, and the basic group of the locus is A or C. By optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the residual feed intake of boars can be reduced, the excellent boars with the characters can be bred, and the genetic improvement progress of the boars can be accelerated, so that the economic benefit of breeding of the boars can be effectively improved.

Description

technical field [0001] The invention relates to a SNP molecular marker on pig No. 1 chromosome related to pig residual feed intake and use thereof. Background technique [0002] Pork is an important meat source for humans, accounting for about 40% of the meat consumed by the world's population. Affected by Chinese meat consumption habits, my country's demand for pork is even greater. Since the 1980s, pork has been the main meat on the tables of urban and rural residents, and pork consumption by urban and rural residents has always accounted for more than 60% of meat consumption, occupying a dominant position. In 2020, the total number of pigs slaughtered in my country will be 527 million heads, accounting for half of the world's pigs slaughtered; pork production will be 41.13 million tons, accounting for 42.02% of global pork production, also ranking first. Pork consumption is irreplaceable, and its safety and effective supply are not only major issues related to the vital...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122Y02P60/87
Inventor 杨杰吴珍芳丁荣荣郑恩琴吴杰蔡更元洪林君杨化强黄思秀
Owner SOUTH CHINA AGRI UNIV
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