Application of Asterothamnus centrali-asiaticus in biological prevention and control, biological prevention and control medicament and preparation method of biological prevention and control medicament
A kind of technology of aster and aster wood, applied to the application of central Asian aster wood in biological control and its biological control agent and preparation field
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[0064] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method of the biological control agent includes: using the essential oil of Asteria chinensis as the active ingredient of the biological control agent.
[0065] In some embodiments, the essential oil of Asteria chinensis is the essential oil extracted from the aerial part of Asteria chinensis plant;
[0066] Preferably, the aerial part of the Asteria chinensis plant is extracted by steam distillation and then extracted;
[0067] Preferably, the extraction temperature of the steam distillation method is 70-120°C, preferably 100°C; the extraction time is 3-9h, preferably 6h;
[0068] Preferably, the extractant is n-hexane, and the extraction time is 2-6h, preferably 4h;
[0069] Preferably, the extracted n-hexane layer is dried and concentrated; preferably, the drying refers to removing the moisture of...
experiment example
[0086] Experimental example, preparation and verification of insecticidal effect of Aster wood essential oil of the present invention
[0087] 1. Plant essential oil preparation
[0088] Take the aboveground part of the plant of Asteria chinensis, dry it naturally in the shade, crush it, put it in a volatile oil extractor after weighing, and extract it by steam distillation. The extraction time is generally 6 hours, and extract it with n-hexane to obtain the n-hexane layer. Add Remove water with anhydrous sodium sulfate, concentrate, measure the volume, put the volatile oil into a dry glass bottle, and store it in a refrigerator at 4°C.
[0089] 2. GC-MS test
[0090] The volatile oil samples were analyzed by GC-MS. The test conditions were: GCMS-QP2010 plus (Shimadzu); Chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); Heating rate: 60°C-10 ℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃;...
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