40 results about "Aphis serpylli" patented technology

The invention relates to a method for large-quantity propylea japonica indoor raising. Wheat aphids, melanaphis sacchari and corn leaf aphids are used as aphids for feeding propylea japonica, and through specific technical measures such as host young aphid cultivating, aphid expanding propagation and propylea japonica artificial feeding, a large quantity of field-preventing and indoor-theory-study propylea japonica is obtained by expanding propagation. According to the method, wheat aphid feeding is carried out at a larval phase, and wheat aphid, melanaphis sacchari and corn leaf aphid feeding is carried out at an adult spawning period. The nutritional requirements of propylea japonica are improved, and the fecundity of propylea japonica is greatly improved. During raising, corn seedlings are placed, the habitat of propylea japonica is enlarged, space complexity is improved, propylea japonica autotomy and competition are lowered, and raising density can be greatly improved. The method is summarized according to the habit of propylea japonica and is easy to operate, economical and practical. Propylea japonica obtained by expanding propagation is high in survival rate and high in fecundity, and large-scale artificial breeding of propylea japonica can be achieved.

The present invention relates to a Cucumis melo seed designated 34-309 RZ, which may exhibit small leaves with erect to semi-erect petioles, a yellow ground color of the fruit skin, fruits that have a medium rate of change of fruit color from maturity to overmaturity, resistance to aphids (Aphis gossypii), intermediate resistance to powdery mildew Podosphaera xanthii (Px) race 1, race 2, race 5, intermediate resistance to powdery mildew Golovinomyces cichoracearum (Gc) race 1. The present invention also relates to a Cucumis melo plant produced by growing the 34-309 RZ seed. The invention further relates to methods for producing the melon cultivar, represented by melon variety 34-309 RZ.

The invention discloses a dsRNA nano nucleic acid insecticide for preventing and treating piercing-sucking pests, the dsRNA nano nucleic acid insecticide is composed of dsRNA of a specific targeting piercing-sucking pest Chs gene and a carrier, the piercing-sucking pests are Panonychus citri, Diaphorina citri or Aphis gossypii, the nucleotide sequences of the Chs gene are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and the carrier is MgAl or MgFe layered double-metal hydroxide nanoparticles. The dsRNA nano nucleic acid insecticide is applied to host plants of the piercing-sucking pests, after the plants induce the piercing-sucking pests to eat the dsRNA nano nucleic acid insecticide, RNA synthesis and silent Chs genes are interfered in vivo, death is promoted, and the dsRNA nano nucleic acid insecticide has good application prospects in treatment of the piercing-sucking pests.

The present invention relates to isolated Vat genes encoding a polypeptide that confers resistance to Aphis gossypii and / or resistance to viral transmission by aphids, and methods of transforming plant and cells with a Vat gene. The invention also relates to transgenic plants and cells having resistance to Aphis gossypii and / or resistance to viral transmission by aphids.

The invention provides a botanical insecticide. The botanical insecticide comprises, by weight, 6-12 parts of ricinus communis, 5-7 parts of zinnia elegans, 3-4 parts of radix saposhnikoviae, 7-10 parts of folium artemisiae argyi, 8-12 parts of radix gentianae, 8-11 parts of radish leaves, 15-20 parts of crassocephalum crepidioides, 13-18 parts of cassava meal, 3-5 parts of Chinese chives, 3-8 parts of brown sugar, 5-9 parts of radix aconiti kusnezoffii and 30-48 parts of isopropyl alcohol. The botanical insecticide has the advantages that reaction is carried out on the brown sugar and the zinnia elegans, and accordingly excellent effects of preventing and treating cucumber bacterial leaf spot and gray mold can be realized; good effects of killing aphids and treating diseases can be realized by the added Chinese chives and the added crassocephalum crepidioides, wound healing can be promoted, and the botanical insecticide further can be used for disinfecting soil.

The invention discloses a medicine formula for preventing and treating Bactrocera minax, and the medicine comprises the following components in parts by weight: 40-60 parts of rheum officinale, 20-40 parts of polygonum multiflorum, 10-20 parts of radix achyranthis bidentatae, 5-15 parts of Phytolacca acinosa, 10-20 parts of Ageratina adenophora, 5-15 parts of Tripterygium wilfordii and 3-12 parts of common andrographis herb; and the botanical insecticide disclosed by the invention has significant effects on aphid, red spiders, borers and fruit flies on fruit trees.

The invention discloses a compound insecticide of lecanicillium lecanii and stemonine and application of the compound insecticide. The compound insecticide is prepared from the following components in percentage by mass: 10-30% of a main agent and a 70-90% of an auxiliary agent, wherein the main agent is a mixture of the lecanicillium lecanii and stemonine; and a mass ratio of the lecanicillium lecanii to the stemonine is 10:1. The compound insecticide disclosed by the invention can be processed into wettable powder and has excellent effects of efficiently controlling injurious insects such as aleyrodid and aphid.

The invention discloses an imidacloprid wettable powder seed-dressing agent for preventing and controlling Aphis gossypii Glover and a preparation method and application thereof and relates to the field of agricultural pharmacology. According to the seed-dressing agent, imidacloprid serving as a main active ingredient, humic acid serving as a synergis, as well as a suspending agent, a wetting agent, an anti-flocculating agent, a carrier and a filling material are fully and uniformly mixed by a jet mill, thus preparing the imidacloprid wettable powder seed-dressing agent for preventing and controlling Aphis gossypii Glover. The prevention and control effect of the imidacloprid wettable powder seed-dressing agent on Aphis gossypii Glover can be over 80 percent, the effect lasting period is about 60 days, and the imidacloprid wettable powder seed-dressing agent has an effect of obviously promoting growth and development of cotton. Meanwhile, the imidacloprid wettable powder seed-dressing agent has the characteristics of high safety, low residue content, low price, high selectivity and the like.

The invention discloses a plant drug for preventing spinach beet aphids, comprising cortex phellodendri powder, wood ashes, sophora flavescens powder, pine needle powder, garlic agent, fennel powder, and tea seed meal. According to the invention, the plant drug disclosed herein has the advantages of convenient formula and preparation method, low price of raw materials, simple compatibility, and good control effect, various beneficial microbes and organic fertilizers included in the plant drug have the effect of gaining weight and promoting rapid growth and good insect-proofing effect of spinach beet, and the plant drug has the effect of increasing the yield and effectively solve the problem of excessive residual pesticides in spinach beet.

The disclosure provides a new and distinct hybrid melon variety NUN 16177 MEM as well as seeds and plants and fruits thereof. NUN 16177 MEM is a Cantaloupe non-sutured melon variety of the Golden subtype with long shelf-life, ovate shape, strong orange rind color and moderate orange flesh, comprising resistance to Podosphaera xanthii Race 1, Race 2, Race 5, Fusarium oxysporum melonis Race 0, Race 1, and Race 2, and Aphis gossypii.

The invention discloses a botanical compound insecticide and applications thereof, and belongs to the technical field of botanical pesticides. The botanical compound insecticide is prepared through the following steps: 1) in percentage by weigh, weighting 0.05-0.45% of camptothecin, 12-31% of bamboo tar, 2.1-5.2% of sodium hydroxide, and 63-85% of water; and mixing the camptothecin, sodium hydroxide and the water in a ratio of 1:(0.84-2.08):(118-404), and stirring at a temperature of 50-55 DEG C so as to obtain a single agent I; 2) adding the rest of sodium hydroxide into the bamboo tar, after the obtained mixture is reacted to room temperature, adding the rest of water, and stirring so as to obtain a single agent II; and 3) mixing the single agent I with the single agent II, and adding a pesticide adjuvant, so that a final product is obtained. The botanical compound insecticide has an ideal contact effect on piercing-sucking and chewing mouthpart pests such as rice planthoppers, chilo suppressalis walkers, cotton aphid, aphis craccivora, myzus persicae and the like, so that the insecticide has an effect of growth-promoting and trace element supplementing on crops; and the botanical compound insecticide is low in production cost and easy to use.

The disclosure relates to melon variety NUN 16058 MEM as well as seeds and plants and fruits thereof. NUN 16058 MEM is a Cantaloupe non-sutured, long shelf life melon variety comprising resistance to Fusarium oxysporum f. sp. melonis Race 0, Race 1 and Race 2, Podosphaera xanthii Race 1, Race 2, and Race 5, Golovinomyces cichoracearum (Gc), and Aphis gossypii (Ag).

The invention discloses a DNA bar code standard detection sequence for apocynum venetum aphids and a molecular identification method based on the standard sequence. The DNA bar code standard detectionsequence is shown as SEQ ID NO. 1. According to the invention, by adoption of a reliable DNA molecular identification technology, the method aims to rapidly identify main pests, namely the apocynum venetum aphids in an apocynum venetum field, and has the characteristics of accuracy, rapidness, convenience and low cost; and compared with a traditional morphological characteristic identification method, the method provided by the invention saves time and reduces cost. The method provided by the invention has low requirements on used instruments and equipment, can be carried out in general molecular biology laboratories, is more accurate and reliable than traditional morphological identification, obtains the CO1 sequence of the apocynum venetum aphids for the first time, fundamentally solvesthe problems that the apocynum venetum aphids are difficult to accurately identify and easy to mix with cotton aphids in a conventional apocynum venetum aphid prevention and treatment process, fillsindustrial blank and has important significance.

The invention discloses a method for breeding aphidius gifuensis, which adopts a specific breeding process, adopts specific male parent and female parent domestication with staged humidity and temperature and adopts different subculture schemes to construct a specific aphidius gifuensis breeding method, and aims to solve the problems that the existing aphidius gifuensis is easy to cause hyperparasitism when the number of cotton aphids is low, and the breeding efficiency is low. The cotton aphid is massively propagated, and the prevention and treatment effect is poor. According to the technical scheme, the breeding mode that the female parents and the male parents are alternately subjected to low-temperature domestication has a remarkable influence on the parasitism rate of F5-generation individuals obtained through breeding, compared with a control group, the parasitism rate difference of the aphidius gifuensis is remarkable, and the survival rate in low-temperature feeding and high-temperature feeding is also remarkably influenced; the stress resistance of the aphidius gifuensis can be improved, and the green and sustainable development of the cotton industry can be realized.

The disclosure relates to melon variety NUN 71248 MEM as well as seeds and plants and fruits thereof. NUN 71248 MEM is a Honeydew melon variety comprising resistance to resistance Fusarium oxysporum f. sp. melonis Race 0 and Race 2, Podosphaera xanthii Race 1 (IR), Race 2 (IR), and Race 5 (IR), and Aphis gossypii (Ag).

The invention discloses an insecticidal essential oil of borlotta serilis, which mainly adopts the above-ground part of the plant of borlotta serilis to be naturally dried in the shade, extracts by steam distillation for 6 hours, and then extracts it with n-hexane to obtain n-hexane The alkane layer was prepared by adding anhydrous sodium sulfate to remove water. The invention also provides the application of the bollota essential oil in killing insects, and the bollota essential oil can effectively achieve contact killing of cotton aphids and psyllids.

The invention belongs to the technical field of artificial reproduction of natural enemy insects and biological control of agricultural pests, and discloses a method for mixed feeding of two kinds of aphid parasitic wasps. The method is mainly technically characterized by comprising the following steps: breeding host plants, inoculating and breeding aphids, obtaining and inoculating two kinds of aphid parasitic wasps, collecting the parasitic wasps, removing impurities, and separating the two kinds of parasitic wasps. According to the method for mixed feeding of the two kinds of aphid parasitic wasps, the two kinds of parasitic natural enemies of the aphidius gifuensis and the aphis gossypii are inoculated in different time periods, the parasitic efficiency of the single species of wasps is improved, interspecific competition between adult wasps is avoided, the two kinds of parasitic wasps are collected at the same time, and manpower and material resources are saved.

The present application relates to the field of pesticide insecticide, and particularly to a kind of insecticide composition and its application.The composition includes active ingredient, and the active ingredient includes zaname and spirotetramat, and the mass ratio of zaname and spirotetramat is 2:1~1:3.The composition has good synergistic effect on piercing-sucking pests such as whitefly, resistant cotton aphid, etc., and has excellent quick-acting and persistence, can achieve better control effect than single agent at lower dose, can effectively delay the development of insect resistance, has long-lasting efficacy, wide control spectrum, good safety, environmental protection, low toxicity and sustainability.

The invention relates to the technical field of insect genetic engineering, in particular to a cotton aphid salivary protein gene C002 and application thereof. The nucleotide sequence of the separated cotton aphid C002 gene is as shown in SEQ ID NO: 1, and the sequence of the protein coded by the cotton aphid C002 gene is as shown in SEQ ID NO: 2. The invention further provides application of the cotton aphid C002 gene RNA interference sequence. The sequence has a good silencing effect on the cotton aphid C002 gene. According to the C002 interference sequence-transfected VIGS cotton plant, the number and weight of offspring of cotton aphid are remarkably reduced, and the population development can be effectively controlled. The gene and the protein provided by the invention can be used for development of transgenic insect-resistant plants and biological prevention and control of aphis gossypii glover.

The invention discloses an insecticidal Artemisia chinensis essential oil. The above-ground parts of the plants of Artemisia chinensis are naturally dried in the shade, pulverized, weighed, placed in an essential oil extractor, and extracted by steam distillation. 6h, extracted with n-hexane to obtain n-hexane layer, which was prepared by adding anhydrous sodium sulfate to remove water. The invention also provides the application of the essential oil of Artemisia annua in insecticidal, and the essential oil of Artemisia annua can effectively achieve contact killing of cotton aphids and psyllids.

The invention relates to an AgDoa gene and application thereof in reduction of insecticide resistance of cotton aphid, and belongs to the technical field of biological genetic engineering, the AgDoa gene has a DNA sequence as shown in SEQ ID No: 1, and consists of 1782 nucleotides; the amino acid sequence of the protein coded by the AgDoa gene is shown as SEQ ID No: 2, and the protein consists of 593 amino acids; the AgDoa gene can be used as a target to develop a novel insecticide; the AgDoa gene is knocked down, so that the tolerance of the aphis gossypii glover to the insecticide is reduced, and the aphis gossypii glover can be applied to drug resistance treatment and novel pesticide development.

The invention relates to a method for conducting aphid conservation in winter by means of winter cabbages. The problems that in the prior art, in the vast northern area of the Yangtze River in our country, a greenhouse capable of keeping a constant temperature must be arranged for achieving conservation of aphids of myzus persicae sulzer species, and the investment on building of the greenhouse capable of keeping the constant temperature and facilitates and equipment for temperature constancy is high are solved. Due to the fact that aphid conservation is conducted by means of the winter cabbages, the problem that pathogens utilizing other aphid conservation hosts are propagated to tobaccos through the aphids is solved, and the problem that aphid conservation is conducted by means of other aphid hosts, and then cabbages are utilized as propagation hosts to conduct detoxification on the aphids is solved.

The invention discloses a lemon insecticide. The lemon insecticide comprises, by weight, 40-60 parts of radix bupleuri, 20-40 parts of flos daturae, 10-20 parts of cortex phellodendri, 5-15 parts of phytolacca acinosa, 10-20 parts of rhizoma acori tatarinowii, 5-15 parts of flos carthami and 3-12 parts of radix paeoniae alba. The lemon insecticide has the advantages that the lemon insecticide is a botanical insecticide, does not contain optional chemical pesticide components, is low in toxicity for human and livestock and is environmentally friendly, and insect pests such as nilaparvata lugens, laodelphax striatellus, bemisia tabaci, aphids, bactrocera dorsalis, pomacea canaliculata and wigglers can be prevented and treated by the lemon insecticide.

A new and distinct melon variety NUN 16019 MEM is disclosed as well as seeds and plants and fruits thereof. NUN 16019 MEM is a non-sutured Cantaloupe melon variety with long shelf life comprising resistance to Fusarium oxysporum melonis Race 0, Race 1, and Race 2, Podosphaera xanthii Race 1, Race 2, and Race 5, Golovinomyces cichoracearum Race 1, and Aphis gossypii.

The invention belongs to the technical field of biological control over agricultural pests and particularly relates to a method for breeding male encarsia sophia by using trioxysrietscheli. The methodcomprises the steps of culturing a host plant cotton; culturing primary hosts bemisia tabaci and aphis gossypii; culturing secondary hosts; preparing female parents of encarsia sophia; breeding maleencarsia sophia. The method achieves the purpose of rapidly producing male encarsia sophia under the condition that there are few inset breeding chambers and the population number of bemisia tabaci ina field and specific parasitic wasps thereof is small, the mass propagation of encarsia sophia before the peak period of bemisia tabaci is thus realized, and the method is used for biological controlover bemisia tabaci.

The invention discloses insecticidal Artemisia giraldii Pamp. essential oil which is prepared by the steps of naturally drying the overground part of an Artemisia giraldii Pamp. plant in the shade, crushing, weighing, putting into an essential oil extractor, extracting for 6 hours by adopting a steam distillation method, extracting by adopting normal hexane to obtain a normal hexane layer, and adding anhydrous sodium sulfate to remove water. The invention also provides an application of the Artemisia giraldii Pamp. essential oil in insect killing. The Artemisia giraldii Pamp. essential oil can effectively realize contact killing of cotton aphids and phylloxera.

The invention discloses a method for identifying the yellow dwarf resistance of triticale. The method comprises the following steps that 1, the triticale is sown in ten rows, and an arched shed is subjected to heat preservation; 2, authigenic triticale seedlings carrying yellow dwarf viruses and plants with yellow dwarf virus symptoms are transplanted into the arched shed, and the typical disease plants are inoculated with aphids for expanding propagation; 3, sowing is carried out, triticale materials are identified, and the materials are sequentially arranged in a field according to identification numbers; 4, when the identified materials are at an elongation stage, disease leaves of the triticale where the yellow dwarf viruses are subjected to expanding propagation are subjected to virus feeding with the aphids, and the identified materials are inoculated with the disease leaves; 5, resistance evaluation is carried out. The method for identifying the yellow dwarf resistance of the triticale is convenient to operate, simple, easy to implement and low in cost, the trouble of indoor storage and aphid reproduction is saved, the resistance of hundreds of triticale materials to all epidemic strains of the local yellow dwarf viruses can be identified at a time, high pertinency is achieved, the detection efficiency is high, and good accuracy is achieved.

The disclosure provides a new and distinct hybrid melon variety NUN 76207 MEM as well as seeds and plants and fruits thereof. NUN 76207 MEM is a Cantaloupe non-sutured melon variety of the Harper subtype with long shelf-life, oval shape, moderate yellow green rind color and dark orange flesh comprising resistance to Podosphaera xanthii Race 1, Race 2, Race 5, Fusarium oxysporum melonis Race 1, Muskmelon Necrotic Spot Virus (MNSV), and Aphis gossypii.

A method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii is provided, relating to the technical field of cotton safety evaluation. The method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii includes planting the gene-edited low-gossypol cotton indoors, collecting young leaves and inoculating nymphal Aphis gossypii on the leaves, rearing the nymphal Aphis gossypii, recording a number of adult Aphis gossypii deaths and a number of newly hatched nymphal Aphis gossypii, calculating an Aphis gossypii mortality rate and an average Aphis gossypii productivity, and evaluating the influence.