An insecticidal essential oil of Artemisia annua and its application
A technology of wormwood and essential oil, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, and achieve the effect of easy-to-obtain, simple reagents and strong drug properties
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Embodiment 1
[0015] This example provides the preparation process and GC-MS test of Artemisia annua essential oil.
[0016] The aerial parts of Artemisia chinensis plants were naturally dried in the shade, pulverized, weighed 500g and placed in an essential oil extractor, extracted by steam distillation, and the distillation time was 6h. The distillate was extracted with n-hexane to obtain a n-hexane layer, anhydrous sodium sulfate was added to remove water, after concentration, the volume was measured, the essential oil was put into a dry glass bottle, and placed in a 4°C refrigerator for storage, that is, the North China Rice Artemisia essential oil.
[0017] The essential oil samples of Artemisia annua were analyzed by GC-MS. The test conditions were: GCMS-QP2010plus (Shimadzu); chromatographic column: DB-5MS (60m×0.25μm×0.32mm) (Agilent); heating rate: 60°C -10℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; i...
Embodiment 2
[0027] This example provides the determination of the contact killing activity of Artemisia annua essential oil on cotton aphid and psyllid.
[0028] (1) Determination of contact killing activity of Artemisia annua essential oil on cotton aphid
[0029] The insecticidal activity was determined by the leaf disc method. Polytetrafluoroethylene was applied to the four walls of a 12-well plate. After drying, the agar was spread on the bottom of the plate. Fresh cotton leaves without pesticides were taken, and the leaves were punched to make a A circular leaf disc with a diameter of 3cm, the leaves are soaked in medicine for 10s, then air-dried and placed in the orifice plate, inserted with 10 wolfberry cotton aphids, and placed in a light / dark=16h / 8h, 25℃, humidity of 60%~70% in the incubator. Negative and positive controls were used without the addition of Artemisia annua essential oil solvent and pyrethrin. Each treatment was repeated 6 times, with 10 cotton aphids per repetit...
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