A kind of insecticidal Bolota serica essential oil and its application
A technology of essential oils and insecticides, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, etc., and achieve the effects of easy-to-obtain reagents, simple production methods, and simple reagents
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Embodiment 1
[0015] The present embodiment provides a preparation process of Artemisia Bolota artemisia sericin essential oil and GC-MS testing.
[0016] The aerial part of the plant is naturally dried, crushed, weighed 500g and placed in a volatile oil extractor, and extracted by water vapor distillation method, and the distillation time is 6h. The distillate is extracted using n-hexane, a layer of n-hexane is obtained, anhydrous sodium sulfate is added to remove the water, after concentration, the volume is taken, the volatile oil is loaded into a dry glass bottle, placed in a refrigerator at 4 ° C for preservation, that is, the essential oil of Artemisia annua bolotta.
[0017] The Bolota Artemisia sycamore essential oil sample was analyzed by GC-MS, and the test conditions were: GCMS-QP2010plus (Shimadzu); column: DB-5MS(60m×0.25um×0.32mm) (Agilent); heating rate: 60°C~10°C / min-300°C-20min; shunt ratio: 30:1; constant line speed mode: 30cm / sec; carrier gas: He; ion source temperature: 200°...
Embodiment 2
[0029] The present embodiment provides a assay of the touch activity of Artemisia bolta artemisia sativa essential oil on cotton aphids and pyllids.
[0030] (1) Determination of the touch killing activity of Artemisia bolta sylvestris essential oil on cotton aphids
[0031] Determination by leaf dish method, PTFE is coated to the four walls of the 12-well plate, after drying, the agar is spread to the bottom of the plate, the fresh cotton leaves without pesticides are taken, the leaves are punched, and a circular leaf dish with a diameter of 3cm is made, the leaves are soaked for 10 seconds and then dried into the leaves, and 10 goji cotton aphids are inserted, placed in an incubator with light / dark = 16h / 8h, 25 °C, humidity of 60% to 70%. The unused essential oil solvent and pyrethroids were controlled negative and positive, respectively. Each treatment was repeated 6 times, each repeat was 10 cotton aphids, and the death and survival of the cotton aphids of each treatment were ...
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