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A kind of insecticidal Bolota serica essential oil and its application

A technology of essential oils and insecticides, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, etc., and achieve the effects of easy-to-obtain reagents, simple production methods, and simple reagents

Active Publication Date: 2022-05-31
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the insecticidal activity of Bolota Seraphim in terms of plant-derived pesticides, especially the insecticidal activity of Bolota Seraphim

Method used

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  • A kind of insecticidal Bolota serica essential oil and its application
  • A kind of insecticidal Bolota serica essential oil and its application
  • A kind of insecticidal Bolota serica essential oil and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The present embodiment provides a preparation process of Artemisia Bolota artemisia sericin essential oil and GC-MS testing.

[0016] The aerial part of the plant is naturally dried, crushed, weighed 500g and placed in a volatile oil extractor, and extracted by water vapor distillation method, and the distillation time is 6h. The distillate is extracted using n-hexane, a layer of n-hexane is obtained, anhydrous sodium sulfate is added to remove the water, after concentration, the volume is taken, the volatile oil is loaded into a dry glass bottle, placed in a refrigerator at 4 ° C for preservation, that is, the essential oil of Artemisia annua bolotta.

[0017] The Bolota Artemisia sycamore essential oil sample was analyzed by GC-MS, and the test conditions were: GCMS-QP2010plus (Shimadzu); column: DB-5MS(60m×0.25um×0.32mm) (Agilent); heating rate: 60°C~10°C / min-300°C-20min; shunt ratio: 30:1; constant line speed mode: 30cm / sec; carrier gas: He; ion source temperature: 200°...

Embodiment 2

[0029] The present embodiment provides a assay of the touch activity of Artemisia bolta artemisia sativa essential oil on cotton aphids and pyllids.

[0030] (1) Determination of the touch killing activity of Artemisia bolta sylvestris essential oil on cotton aphids

[0031] Determination by leaf dish method, PTFE is coated to the four walls of the 12-well plate, after drying, the agar is spread to the bottom of the plate, the fresh cotton leaves without pesticides are taken, the leaves are punched, and a circular leaf dish with a diameter of 3cm is made, the leaves are soaked for 10 seconds and then dried into the leaves, and 10 goji cotton aphids are inserted, placed in an incubator with light / dark = 16h / 8h, 25 °C, humidity of 60% to 70%. The unused essential oil solvent and pyrethroids were controlled negative and positive, respectively. Each treatment was repeated 6 times, each repeat was 10 cotton aphids, and the death and survival of the cotton aphids of each treatment were ...

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Abstract

The invention discloses an insecticidal essential oil of borlotta serilis, which mainly adopts the above-ground part of the plant of borlotta serilis to be naturally dried in the shade, extracts by steam distillation for 6 hours, and then extracts it with n-hexane to obtain n-hexane The alkane layer was prepared by adding anhydrous sodium sulfate to remove water. The invention also provides the application of the bollota essential oil in killing insects, and the bollota essential oil can effectively achieve contact killing of cotton aphids and psyllids.

Description

Technical field [0001] The present invention belongs to the field of plant-derived pesticide technology, relates to an artemisia terrestris plant, specifically to an insecticidal bolotta artemisia terrestris essential oil and its applications. Background [0002] With the continuous deepening of pesticide research, plant-derived pesticides have made great progress in recent years, which has the advantages of safety for humans and livestock, does not pollute the environment, is not easy to cause resistance, and is easy to degrade in the natural environment. Plant-derived pesticides mainly include active ingredients extracted from plants, plants themselves and compounds and derivatives synthesized according to the active structure. For example, neem, which has been industrially produced, is a secondary metabolite obtained from the neem plant socks; the currently widely used natural insecticide monothrin insecticide is developed from the pyrethroid modification in the pyrethroid of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P7/04
CPCA01N65/12A01N65/00Y02A50/30
Inventor 刘畅魏淑花王芳张蓉何嘉黄文广王颖朱蒙猛宋双
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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