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The method for breeding the drone of A. gossypis by using the short tuberculosis wasp

A technology of E. aphid males and E. pale yellow wasps, which is applied in the direction of animal husbandry and the like, can solve the problems of late occurrence period, low reproduction amount of female species, stable number and the like, and achieves easy operation, good control effect, easy obtained effect

Active Publication Date: 2020-12-11
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing method of using the secondary host of A. haideni to breed the drone of A. japonica, there are problems that the secondary host is not easy to obtain, the occurrence period is late, and the reproductive capacity of the female bee is low. These problems are directly related to As a result, during the outbreak period of whitefly, it is impossible to provide sufficient and stable numbers of Aphidia japonica drones, which seriously affects the control effect

Method used

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  • The method for breeding the drone of A. gossypis by using the short tuberculosis wasp
  • The method for breeding the drone of A. gossypis by using the short tuberculosis wasp
  • The method for breeding the drone of A. gossypis by using the short tuberculosis wasp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Propagation of the Drone Aphidia spp.

[0025] (1) Cultivation of host plant cotton

[0026] Cotton seeds are sown in a seedling tray with a nutrient substrate for cultivation, 1 seed / hole, and the nutrient substrate is composed of vermiculite, flower nutrient soil and soil; the volume ratio of vermiculite: flower nutrient soil: soil is 1 -1.5:2-4:3-5; Regularly check the germination of cotton seeds and the growth of seedlings, and maintain the humidity of the nutrient matrix in the seedling tray. When the cotton seedling plant grows to 1-2 true leaves, transplant it to a nutrient cup with a nutrient substrate for cultivation, 1 plant / cup, and wait for the cotton seedling plant to grow to 3-4 true leaves;

[0027] The cultivation of the host plant cotton is carried out in a greenhouse with sufficient light and controllable environmental conditions to ensure no pollution by diseases and insect pests, the temperature is 26±2°C, the relative humidity is 70-75%, ...

Embodiment 2

[0039] Example 2 Comparison of fecundity of male bees bred by the parasitic Aphidia breviparasitic Aphids aphids and Aphidia haidenii

[0040] 2.1 Insects to be tested

[0041] Host insect: Bemisia tabaci (Gennadius) type B

[0042]Parasitic wasps: Encarsia sophia (Girault&Dodd); Trioxysrietscheli (Mackauer); Eretmocerus hayati (Zolnerowich&Rose).

[0043] 2.2 Tested host plants

[0044] Cotton Gossypium spp (Hebei Zhongchuang Nongke Seed Industry Technology Co., Ltd., Xinke No. 8). The potted cotton is used when it grows to 4 true leaves.

[0045] 2.3 Experimental method

[0046] (1) Take cotton seedlings containing 50 2-3 instar cotton aphid nymphs and put them into insect cages, insert 5 pairs of A. When the wasp wasp developed to 2 days old, 30 unmated virgin female wasps of E. flavus were inoculated, and removed after 24 hours.

[0047] At the same time, the cotton seedlings carrying the 3rd-instar Bemisia tabaci nymphs were put into insect cages, and 5 pairs of A. ...

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Abstract

The invention belongs to the technical field of biological control over agricultural pests and particularly relates to a method for breeding male encarsia sophia by using trioxysrietscheli. The methodcomprises the steps of culturing a host plant cotton; culturing primary hosts bemisia tabaci and aphis gossypii; culturing secondary hosts; preparing female parents of encarsia sophia; breeding maleencarsia sophia. The method achieves the purpose of rapidly producing male encarsia sophia under the condition that there are few inset breeding chambers and the population number of bemisia tabaci ina field and specific parasitic wasps thereof is small, the mass propagation of encarsia sophia before the peak period of bemisia tabaci is thus realized, and the method is used for biological controlover bemisia tabaci.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pests, and in particular relates to a method for breeding drones of A. Background technique [0002] Whitefly Bemisia tabaci (Gennadius) is a very serious worldwide pest. There are more than 600 species of host plants, and it can carry more than 70 plant viruses. It is seriously harmful to cotton, vegetables and flowers in my country. The wasp Encarsia sophia is the main parasitoid of Bemisia tabaci, and it is distributed in both southern and northern my country. The bee belongs to the order Hymenoptera, Aphididae, and the genus Aphididae. It is a monoparasitic endoparasitoid and has a unique facultative autoparasitic reproductive mode, that is, the female bee is a primary parasitoid and parasitizes on the primary host. Bemisia tabaci nymphs, while drones are polyparasitoids, which parasitize on the same or heterogeneous parasitoid larvae of the secondary host. Under n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 杨念婉满孝明刘万学李茂海宁晓雪刘赞赵雪杨徐海云万方浩
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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