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Application of Aster indicus in field of biocontrol, biocontrol agent, preparation method of biocontrol agent and insect killing method

A Malan, the use of technology, applied in biological control, Malan Asterindicus in the field of biological control, can solve the use of Malan has not been reported and other problems

Active Publication Date: 2021-11-30
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report about the use of Malan in the field of biological control

Method used

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  • Application of Aster indicus in field of biocontrol, biocontrol agent, preparation method of biocontrol agent and insect killing method
  • Application of Aster indicus in field of biocontrol, biocontrol agent, preparation method of biocontrol agent and insect killing method
  • Application of Aster indicus in field of biocontrol, biocontrol agent, preparation method of biocontrol agent and insect killing method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: using Malan essential oil as the active ingredient of the biological control agent.

[0061] In a specific embodiment, essential oil is extracted from the aerial part of Malan;

[0062] Preferably, the aerial part of Malan is extracted with an extractant; preferably, the extractant is n-hexane, and the extraction time is 4-12h, preferably 6h;

[0063] Preferably, the aerial part of Malan is dried in the shade and pulverized before extraction; the extraction refers to placing the aerial part of Malan in a volatile oil extractor for extraction;

[0064] Preferably, the n-hexane layer obtained after extraction is dried and concentrated; preferably, the drying refers to adding anhydrous sodium sulfate to the n-hexane layer to remove moisture; preferably, the concentration refers to evaporation of the dried product by a r...

experiment example

[0075] Experimental example, the preparation of Malan essential oil of the present invention and its effect verification

[0076] 1. Plant essential oil preparation

[0077] Take the above-ground part of the Malan plant and dry it naturally, crush it, weigh it, place it in a volatile oil extractor, and extract it by steam distillation. The extraction time is generally 6 hours, and it is extracted with n-hexane to obtain the n-hexane layer, and then add anhydrous sulfuric acid Remove water from sodium, concentrate, measure the volume, put the volatile oil into a dry glass bottle, and store it in a refrigerator at 4°C.

[0078] 2. GC-MS test

[0079] The volatile oil samples were analyzed by GC-MS. The test conditions were: GCMS-QP2010 plus (Shimadzu); Chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); Heating rate: 60°C-10 ℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; interface temperat...

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Abstract

The invention discloses application of Aster indicus in the field of biocontrol, a biocontrol agent, a preparation method of the biocontrol agent and an insect killing method, and belongs to the field of biocontrol. The invention provides application of Aster indicus in the field of biocontrol, and further provides a biocontrol agent based on the application. The biocontrol agent comprises an active component, and the active component comprises Aster indicus essential oil. The invention also provides a preparation method of the biocontrol agent, which is characterized in that the Aster indicus essential oil is used as an active component of the biocontrol agent. The invention further provides an insect killing method which is characterized in that the Aster indicus essential oil is adopted for killing insects. By utilizing the new application of the Aster indicus or adopting the insecticide / insect killing method disclosed by the invention, Frankliniella occidentalis, cotton aphid or phylloxera can be efficiently killed. The Aster indicus is a wild plant, is wide in distribution range, is simple and easy to obtain, is low in cost and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to the use of Malan Aster indicus in the field of biological control, its biological control agent, its preparation method and its insecticidal method. Background technique [0002] Malan Aster indicus is a plant of the genus Aster in the phylum Angiospermae, Compositae Asteraceae. Its whole plant or root can be used as medicine. The plant mostly grows in moist areas of low mountains, flat dams or hills. Distributed in various provinces in southern my country. [0003] Malan nature and flavor are pungent, bitter, cold, function cures mainly: heat-clearing and toxic substances removing, blood stasis dispelling and hemostasis, eliminating stagnation. For cold and fever, cough, acute pharyngitis, tonsillitis, mumps, infectious hepatitis, gastric and duodenal ulcers, infantile malnutrition, enteritis, dysentery, hematemesis, uterine bleeding, irregular menstruat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/12A01P7/00A01P7/04
CPCA01N65/00A01N65/12Y02A50/30
Inventor 刘畅马建华张治科白小军周涛许泽华王颖李百云郭鑫年王贵荣
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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