The application of rosa sinensis in biological control and its biological control agent and preparation method
A technology of saffron and medicament, which is applied in the field of biological control and can solve the problems of unreported use of saury
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[0051] The examples in this group provide a method for preparing a biological control agent. All the examples in this group have the following common features: the preparation method of the biological control agent comprises: extracting essential oil from S. chinensis.
[0052] In a specific embodiment, the Nanmuxiang refers to the root of Aristolochia yunnanensis plant;
[0053] Preferably, the essential oil extraction refers to extraction by steam distillation and then extraction.
[0054] In some embodiments, the extraction temperature of the steam distillation method is 70-120°C, preferably 100°C; the extraction time is 2-8h, preferably 6h;
[0055] Preferably, the extraction agent is n-hexane, and the extraction time is 0.2-1h, preferably 0.5h;
[0056] Preferably, the extracted n-hexane layer is dried and concentrated; preferably, the drying refers to removing the moisture of the n-hexane layer with anhydrous sodium sulfate; the concentration refers to that the dried p...
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[0059] Experimental example, specific preparation process, component identification and effect verification of the biological control agent of the present invention
[0060] 1. Preparation of plant essential oils
[0061] The roots of Nanmuxiang plants were naturally dried in the shade, pulverized, placed in a volatile oil extractor after weighing, and extracted by steam distillation. The extraction time was generally 6h, and n-hexane was used for extraction to obtain a n-hexane layer, and anhydrous sulfuric acid was added. Sodium to remove water, after concentration, measure the volume, put the volatile oil into a dry glass bottle, and store it in a 4°C refrigerator.
[0062] 2. GC-MS test
[0063] The volatile oil samples were analyzed by GC-MS. The test conditions were: GCMS-QP2010 plus (Shimadzu); chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); heating rate: 60℃-10 ℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He...
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