Application of Aristolochia yunnanensis Franch in biological prevention and control, biological prevention and control agent and preparation method of biological prevention and control agent
A kind of technology of fenugreek, which is applied in the field of biological control, can solve the problems such as unreported uses of fenugreek
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[0051] The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method of the biological control agent includes: extracting the essential oil of nanmuxiang.
[0052] In a specific embodiment, said Nanmuxiang refers to the root of Aristolochia yunnanensis plant;
[0053] Preferably, the essential oil extraction refers to extracting by steam distillation and then extracting.
[0054] In some embodiments, the extraction temperature of the steam distillation method is 70-120°C, preferably 100°C; the extraction time is 2-8h, preferably 6h;
[0055] Preferably, the extractant is n-hexane, and the extraction time is 0.2-1h, preferably 0.5h;
[0056] Preferably, the extracted n-hexane layer is dried and concentrated; preferably, the drying refers to removing the moisture of the n-hexane layer with anhydrous sodium sulfate; Get Southernwood Essential Oil;
[0057] Prefe...
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[0059] Experimental example, the specific preparation process of the biological control agent of the present invention, component identification and effect verification
[0060] 1. Plant essential oil preparation
[0061] The root of the plant is naturally dried in the shade, crushed, weighed, placed in a volatile oil extractor, extracted by steam distillation, the extraction time is generally 6 hours, extracted with n-hexane to obtain the n-hexane layer, and added with anhydrous sulfuric acid Remove water from sodium, concentrate, measure the volume, put the volatile oil into a dry glass bottle, and store it in a refrigerator at 4°C.
[0062] 2. GC-MS test
[0063] The volatile oil samples were analyzed by GC-MS. The test conditions were: GCMS-QP2010 plus (Shimadzu); Chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); Heating rate: 60°C-10 ℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; i...
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