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Grafting and mutagenesis combined macadamia nut breeding method

A combined and nut-based technology, applied in the field of macadamia nut breeding combining grafting and mutagenesis, can solve the problems that tissues or organs are difficult to independently cultivate into seedlings, and tissue rapid propagation technology is immature, so as to enhance seed germination, shorten The effect of germination time and strong adaptability

Active Publication Date: 2021-12-31
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the tissue rapid propagation technology of macadamia nuts is immature, and it is difficult to independently cultivate the mutated tissues or organs into seedlings

Method used

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  • Grafting and mutagenesis combined macadamia nut breeding method
  • Grafting and mutagenesis combined macadamia nut breeding method

Examples

Experimental program
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Effect test

Embodiment 1

[0051] A macadamia breeding method combining grafting and mutagenesis, comprising the following steps:

[0052] Step S1, seed selection: picking mature macadamia nut seeds, peeling them manually, and selecting shelled macadamia nuts with a smooth shell surface, no pests and diseases, and no damaged shells as seeds;

[0053] Step S2, sterilizing treatment: sterilizing the seeds to obtain sterilized seeds; the sterilizing solution uses water as a solvent and contains spinosad at a concentration of 10 g / kg and thiophanate-methyl at a concentration of 30 g / kg;

[0054] Step S3, shock processing:

[0055] Specifically include the following steps:

[0056] Step S31, soaking the sterilized seeds in deionized water for 20 minutes, draining the water, and vibrating them for 20 minutes at a frequency of 60 times per second to obtain seeds treated with one shock;

[0057] Step S32, putting the seeds subjected to the primary shock treatment into the pretreatment solution for 30 minutes,...

Embodiment 2

[0072] A macadamia breeding method combining grafting and mutagenesis, comprising the following steps:

[0073] Step S1, seed selection: picking mature macadamia nut seeds, peeling them manually, and selecting shelled macadamia nuts with a smooth shell surface, no pests and diseases, and no damaged shells as seeds;

[0074] Step S2, sterilizing treatment: sterilizing the seeds to obtain sterilized seeds; the sterilizing solution uses water as a solvent and contains spinosad at a concentration of 15 g / kg and thiophanate-methyl at a concentration of 35 g / kg;

[0075] Step S3, shock processing:

[0076] Specifically include the following steps:

[0077] Step S31, soaking the sterilized seeds in deionized water for 30 minutes, draining the water, and shaking them for 25 minutes at a frequency of 80 times / second to obtain the seeds shaken once;

[0078] Step S32, putting the seeds subjected to the primary shock treatment into the pretreatment solution for 45 minutes, draining the...

Embodiment 3

[0093] A macadamia breeding method combining grafting and mutagenesis, comprising the following steps:

[0094] Step S1, seed selection: picking mature macadamia nut seeds, peeling them manually, and selecting shelled macadamia nuts with a smooth shell surface, no pests and diseases, and no damaged shells as seeds;

[0095] Step S2, sterilizing treatment: sterilizing the seeds to obtain sterilized seeds; the sterilizing solution uses water as a solvent and contains spinosad at a concentration of 12 g / kg and thiophanate-methyl at a concentration of 32 g / kg;

[0096] Step S3, shock processing:

[0097] Specifically include the following steps:

[0098] Step S31, soaking the sterilized seeds in deionized water for 25 minutes, draining the water, and shaking them for 22 minutes at a frequency of 65 times per second to obtain the seeds shaken once;

[0099] Step S32, putting the seeds subjected to the primary shock treatment into the pretreatment solution for 35 minutes, draining...

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Abstract

The invention discloses a grafting and mutagenesis combined macadamia nut breeding method. According to the grafting and mutagenesis combined macadamia nut breeding method, macadamia nut seeds are subjected to vibration treatment for three times, the growth capacity and the adaptive capacity of macadamia nut seedlings can be effectively improved, rootstocks obtained through cultivation and used for macadamia nut grafting are high in adaptive capacity, and the survival rate of tender shoots on chemically mutagenized scion strips grafted on the rootstocks is higher; the problem that mutated tender shoots cannot normally grow and develop is effectively solved through a tender shoot grafting technology, and the survival rate of the tender shoots is increased; ultraviolet rays and infrared rays are adopted for alternate irradiation, so that the mutagenesis effect can be greatly improved; a proper amount of polyethylene glycol is added into the mutagenesis paste, so that the effect of enhancing the mutagenesis effect is achieved, the shoot tip part is not prone to distortion, and the mutagenesis effect is good; the scion strips coated with the mutagenesis paste are stored at medium and low temperature, the environment temperature is changed for multiple times, the scion strips are stimulated to mutate, and the mutagenesis effect is better; and n-butyl alcohol is also added into the mutagenesis paste, so that the release of nitrosoethylurea is facilitated, and the effect of enhancing the mutagenesis effect is achieved.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a macadamia breeding method combining grafting and mutagenesis. Background technique [0002] Macadamia nut alias: Queensland chestnut, macadamia walnut, macadamia nut, Queensland fruit, is a tree nut native to Australia. Macadamia nuts are evergreen trees and dicotyledonous plants. The crown is tall, with 3 to 4 leaves in whorls, lanceolate, leathery, smooth, with spiny serrations on the edges. Racemes axillary, beige flowers, spherical fruit, leathery, hard endocarp, beige to light brown kernels. It is suitable for growing in mild, humid and windy areas. Among the many dried fruits in the world, macadamia nuts have the highest economic value and have always enjoyed the reputation of "the king of dried fruits". The kernel of macadamia nuts is rich in nutrition (milky white), the exocarp is turquoise, the endocarp is hard and brown, the weight of a single fruit ...

Claims

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Application Information

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IPC IPC(8): A01G2/30A01G2/38A01H1/06A01C1/00A01C1/08A01N47/28A01N31/02A01N25/04A01N43/22A01N47/34A01P3/00A01P21/00
CPCA01G2/30A01G2/38A01H1/06A01C1/00A01C1/08A01N47/28A01N31/02A01N25/04A01N43/22A01N47/34
Inventor 蔡元保杨祥燕
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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